6.1 The Medicolegal Examination, Physical Evidence & the Crime Scene

Key Takeaways

  • Medicolegal examinations are done by health officers, medical officers of law-enforcement agencies (PNP Forensic Group, NBI) and accredited hospital staff; PD 856 Sec. 95 lists the same three for autopsy.

  • Locard's exchange principle (every contact leaves a trace) is the basis for collecting trace evidence such as hair, fibers, blood and semen.

  • Phenolphthalein (Kastle-Meyer) and luminol are presumptive tests for blood; Teichmann (hemin) and Takayama (hemochromogen) crystals are confirmatory, and the precipitin test shows that blood is human.

  • Acid phosphatase is a presumptive test for semen; spermatozoa on microscopy or prostate-specific antigen (p30) confirm it, and PSA still works when the assailant is azoospermic.

  • Under the Rule on DNA Evidence (A.M. No. 06-11-5-SC), a probability of paternity of 99.9% or more creates a disputable presumption of paternity; chain of custody affects the weight given to DNA results.

Last updated: October 2026

Why this area matters

The 2025 Legal Medicine TOS asks you to "select the appropriate medicolegal examination for the given cases" and "explain the evidence collected from the crime scene". Every physician in the Philippines may be the first to examine an injured, assaulted or dead person, so the PLE treats this as general-practice knowledge, not specialist forensic pathology.

Who performs medicolegal examinations

A medicolegal case is any case in which a physician's findings may be used in a legal proceeding: injuries from violence, sexual assault, poisoning, suspicious or unexplained deaths, deaths in custody, and questions of identity, age or capacity. Examinations are done by:

  • medico-legal officers of the Philippine National Police Forensic Group and the National Bureau of Investigation;
  • municipal and city health officers;
  • hospital physicians, especially in emergency rooms and Women and Children Protection Units.

For autopsy, PD 856 (the Sanitation Code) Section 95 authorizes health officers, medical officers of law-enforcement agencies and members of the medical staff of accredited hospitals.

Kinds of evidence

TypeMeaningMedical examples
Real (autoptic) evidenceThings the court can examine directlyThe wound, a weapon, a bullet, clothing
Testimonial evidenceStatements of witnesses, including expert opinionThe physician's testimony on the cause and age of injuries
Documentary evidenceWritings offered as proofMedicolegal certificate, autopsy report, hospital chart, death certificate
Associative or trace evidenceLinks a suspect to a victim or sceneHair, fibers, blood spatter, semen, soil, fingerprints

Locard's exchange principle: every contact leaves a trace. The suspect takes something from the scene and leaves something behind. This is why victims' fingernail scrapings, clothing and swabs are collected.

The crime scene and chain of custody

  1. Protect the scene: secure the area, limit entry and record everyone who enters.
  2. Document before touching anything: photographs, a sketch with measurements and written notes.
  3. Collect each item separately. Dry wet biological material in air and package it in paper, not plastic, to prevent mold and DNA degradation. Label every package with the date, time, location and collector.
  4. Preserve and transmit evidence with an unbroken chain of custody, meaning a written record of every person who handled it.

A broken chain weakens the evidence. In drug cases, Section 21 of RA 9165 as amended by RA 10640 sets specific inventory and witness requirements.

Examining the living

  • Get informed consent from the patient, or from a parent or guardian for a minor. Explain that findings may be used in court.
  • Take a history in the patient's own words, including time, place, weapon and mechanism.
  • Describe each injury systematically:
    • type (abrasion, contusion, laceration, incised, stab or gunshot wound);
    • exact location measured from fixed anatomical landmarks;
    • size, shape and color;
    • features that suggest age or weapon.
  • Use body diagrams and photographs with a scale.
  • Estimate the healing period. This drives the Revised Penal Code classification as slight, less serious or serious physical injuries.
  • Issue a medicolegal certificate limited to objective findings and opinions within your competence.

Examining the dead

  • External examination: identification, clothing, postmortem changes to estimate time of death, injuries and their distribution.
  • Autopsy: follows PD 856 Section 95, discussed in the death-certification section.
  • Exhumation of buried remains requires legal authority, for example when a prosecutor or the Solicitor General needs to take possession of remains to determine the cause of death.

Laboratory examination of biological evidence

SpecimenPresumptive testsConfirmatory and individualizing tests
BloodPhenolphthalein (Kastle-Meyer), benzidine, luminol (chemiluminescence; shows washed-away stains)Teichmann (hemin crystals), Takayama (hemochromogen crystals), spectroscopy; precipitin test for human origin; ABO grouping; DNA
SemenAcid phosphatase; Florence test (choline crystals); Barberio test (spermine picrate)Spermatozoa on microscopy; prostate-specific antigen (p30), which works even in vasectomized or azoospermic men; DNA
HairGross and microscopic examinationHuman hair has a narrow medulla (less than one-third of shaft width) compared with animal hair. Roots with sheath cells give nuclear DNA; shafts give mitochondrial DNA

Motile spermatozoa may survive in the vagina for only a few hours. Nonmotile sperm are usually detectable for up to about 3 days, and longer in the cervix. Swab early, and record the interval since the alleged assault.

The Rule on DNA Evidence (A.M. No. 06-11-5-SC, 2007)

  • Courts may order DNA testing on application or on their own motion when a biological sample exists, testing would yield relevant results, and the method is scientifically valid.
  • In weighing the result, the court looks at:
    • how the samples were collected, handled and stored, including chain of custody;
    • the possibility of contamination;
    • the procedure and standards followed;
    • the qualifications of the analyst.
  • Paternity: a probability of paternity of 99.9% or higher creates a disputable presumption of paternity. A value below 99.9% is only corroborative evidence. Results that exclude the putative father are treated in jurisprudence (Herrera v. Alba, 2005) as conclusive proof of non-paternity.
  • Convicted persons may seek post-conviction DNA testing when new results could change the verdict.

The physician as witness

An ordinary witness testifies only to facts personally observed, such as "the wound measured 3 cm". An expert witness may give opinions, such as "the wound is consistent with a single-edged blade" or "the injuries will heal in 10 to 30 days", once qualified by training and experience. Under Article III of the PMA Code of Ethics, physicians must assist the government in the administration of justice, and may be fairly paid when called as expert witnesses. A subpoena from a court must be obeyed. Stay objective, testify within your competence and avoid advocacy.

Test Your Knowledge

A dried reddish-brown stain on a suspect's shirt gives a positive phenolphthalein (Kastle-Meyer) test. What should the medicolegal examiner conclude at this stage?

A

The stain is animal blood, because human blood gives a negative result

B

The stain is semen, because the phenolphthalein test reacts with the acid phosphatase found in seminal fluid stains

C

It may be blood, but confirmatory and precipitin tests are needed to prove it is human blood

D

The stain is human blood belonging to the victim

Test Your Knowledge

A woman reports rape by a man who had a vasectomy. Vaginal swabs show no spermatozoa. Which test best demonstrates the presence of seminal fluid?

A

Prostate-specific antigen (p30) on the swab

B

Microscopic search for spermatozoa on a second smear

C

Florence test of the swab for choline crystals

D

Teichmann test for hemin crystals

Test Your Knowledge

Why should wet, blood-soaked clothing from a stabbing victim be air-dried and packed in paper bags rather than sealed plastic bags?

A

Plastic bags absorb blood from the fabric, which removes the stains that are needed for grouping and typing

B

Air-drying converts blood to hemin crystals for the Teichmann test

C

Paper bags are required only because plastic cannot be labeled

D

Moisture trapped in plastic encourages bacterial and fungal growth that degrades DNA and other evidence

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