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100+ Free OAE Biology Practice Questions

Pass your Ohio Assessments for Educators Biology (007) exam on the first try — instant access, no signup required.

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Why does crossing over during prophase I of meiosis increase genetic variation?

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2026 Statistics

Key Facts: OAE Biology Exam

150

Multiple-Choice Questions on Biology (007)

OAE Biology (007) test page

3h

Testing Time

OAE Biology (007) test page

220

Passing Score

OAE Biology (007) test page

$109

Assessment Fee

OAE Biology (007) test page

27%

Largest Current Domain: Genetics and Evolution

OAE Biology (007) assessment framework

Late 2026

Anticipated Testing End Date for Biology (007)

OAE assessments list

Late 2026

Anticipated Testing Start Date for Biology (706)

OAE assessments list and Biology (706) test page

Not reported

Public Pass Rate

OAE/Pearson public pages reviewed

The current OAE Biology (007) test page lists 150 multiple-choice questions, 3 hours of testing time inside a 3 hour 15 minute appointment, a passing score of 220, a $109 assessment fee, computer-based and online-proctored delivery, and an on-screen scientific calculator. The official Biology (007) framework weights Nature of Science at 20%, Biochemistry and Cell Biology at 13%, Genetics and Evolution at 27%, Biological Unity and Diversity at 20%, and Ecology and Environment at 20%. OAE also lists Biology (007) with an anticipated testing end date in late 2026 and Biology (706) with an anticipated testing start date in late 2026, pending State Board approval.

Sample OAE Biology Practice Questions

Try these sample questions to test your OAE Biology exam readiness. Each question includes a detailed explanation. Start the interactive quiz above for the full 100+ question experience with AI tutoring.

1In an experiment testing whether nitrogen fertilizer affects bean plant growth, which setup best identifies nitrogen availability as the independent variable?
A.Several groups receive different nitrogen concentrations while light, water, soil type, and temperature are kept constant
B.One group receives more light and more nitrogen while another receives less water
C.All plants receive the same fertilizer and are measured at the end of the month
D.Plants are grown outdoors without recording rainfall or temperature
Explanation: The independent variable is the factor intentionally changed by the investigator. Holding other conditions constant makes it more defensible to attribute growth differences to nitrogen availability.
2A biology teacher wants students to test whether an antimicrobial disk inhibits bacterial growth. What is the best role of a blank paper disk placed on the same agar plate?
A.It serves as a negative control for the disk material itself
B.It increases the concentration of antimicrobial around the plate
C.It provides nutrients needed for bacterial growth
D.It confirms that all bacteria on the plate are pathogenic
Explanation: A blank disk helps show whether any clear zone is due to the antimicrobial rather than the paper disk or handling. Controls are essential for interpreting experimental results.
3Which statement best describes why repeated trials are important in a biological investigation?
A.They help distinguish a consistent pattern from random variation or measurement error
B.They guarantee that the original hypothesis will be supported
C.They remove the need for a control group
D.They make qualitative observations unnecessary
Explanation: Biological systems vary, and repeated trials improve confidence that a result is not an accident of one sample. Replication strengthens reliability but does not guarantee a particular conclusion.
4Which graph would be most appropriate for showing how dissolved oxygen concentration changes with stream temperature across many sampling sites?
A.A scatterplot with temperature on one axis and dissolved oxygen on the other
B.A pie chart showing the percentage of each fish species
C.A pedigree chart showing inheritance patterns
D.A food web diagram showing feeding relationships
Explanation: A scatterplot is appropriate when comparing two quantitative variables and looking for a relationship. It can show whether dissolved oxygen tends to rise, fall, or show no clear association with temperature.
5A student records a bacterial population as 4.2 x 10^6 cells per milliliter. What does this value mean in standard notation?
A.4,200,000 cells per milliliter
B.420,000 cells per milliliter
C.42,000,000 cells per milliliter
D.0.0000042 cells per milliliter
Explanation: Multiplying by 10^6 moves the decimal point six places to the right. Thus 4.2 x 10^6 equals 4,200,000.
6Which safety practice is most appropriate when students use a compound microscope to observe prepared human blood smears?
A.Treat the slides as biological material and avoid direct contact with the specimen area
B.Remove coverslips to improve magnification
C.Dispose of glass slides in regular classroom trash
D.Focus first with the high-power objective touching the slide
Explanation: Prepared biological slides should be handled carefully, with direct contact with specimen material avoided. Safe microscopy also includes proper slide handling and disposal of broken glass in a sharps container.
7Which statement best distinguishes a scientific theory from a hypothesis?
A.A theory is a broad, evidence-supported explanation, while a hypothesis is a testable proposed explanation for a specific question
B.A theory is an unsupported guess, while a hypothesis is a proven law
C.A theory cannot change, while a hypothesis changes after every observation
D.A theory is used only in physics, while a hypothesis is used only in biology
Explanation: In science, a theory integrates many lines of evidence to explain a broad pattern, such as evolution by natural selection. A hypothesis is narrower and testable in a specific investigation.
8Why does increasing sample size usually strengthen a field study of plant diversity?
A.It reduces the influence of unusual individual plots on the overall estimate
B.It eliminates all sampling bias automatically
C.It changes an observational study into a controlled experiment
D.It makes statistical analysis unnecessary
Explanation: Larger samples generally provide a more stable estimate of a population or community pattern. They do not by themselves fix biased sampling, but they reduce the effect of random variation among individual plots.
9A dataset on seed germination includes one value far higher than all other trials because a student accidentally counted two dishes together. Which measure is most affected by this outlier?
A.Mean
B.Median
C.Mode
D.Sample size
Explanation: The mean uses every numerical value, so an extreme value can pull it upward or downward. The median is often more resistant to a single outlier.
10A student extends a line on a graph beyond the measured range to predict enzyme activity at a much higher temperature. What is the main limitation of this prediction?
A.It is an extrapolation and may be unreliable outside the tested temperature range
B.It is invalid because graphs cannot be used for predictions
C.It proves that enzyme activity increases forever with temperature
D.It is more reliable than collecting additional data
Explanation: Extrapolation estimates beyond the data collected, so it can miss biological changes such as enzyme denaturation. Predictions are strongest when supported by measurements in the relevant range.

About the OAE Biology Exam

The OAE Biology (007) assessment is the current Ohio Assessments for Educators biology content test for educator licensure candidates. The official framework covers nature of science, biochemistry and cell biology, genetics and evolution, biological unity and diversity, and ecology and environment. OAE lists Biology (007) with an anticipated testing end date in late 2026 and Biology (706) with an anticipated testing start date in late 2026.

Questions

150 scored questions

Time Limit

3h 15m appointment (3h testing)

Passing Score

220

Exam Fee

$109 (State Board of Education of Ohio / Pearson)

OAE Biology Exam Content Outline

20% of assessment score

Nature of Science

Scientific inquiry, experimental design, data collection and interpretation, scientific notation, lab safety, the nature and history of science, and evaluation of scientific and technological claims.

13% of assessment score

Biochemistry and Cell Biology

Atoms and molecules, water and carbon, thermodynamics, macromolecules, enzymes, membranes, organelles, cell homeostasis, photosynthesis, cellular respiration, cell specialization, and cell division.

27% of assessment score

Genetics and Evolution

Nucleic acids, gene expression, mutations, genetic engineering, meiosis, inheritance patterns, genetics problems, Hardy-Weinberg equilibrium, natural selection, speciation, fossil evidence, classification, and phylogeny.

20% of assessment score

Biological Unity and Diversity

Characteristics and life cycles of viruses, prokaryotes, protists, fungi, plants, and animals; organismal energy and matter use; homeostasis; and human organ-system anatomy and physiology.

20% of assessment score

Ecology and Environment

Population regulation, behavior, communities, niches, succession, ecosystems, biomes, trophic levels, biogeochemical cycles, and effects of human activity on aquatic, terrestrial, atmospheric, and climate systems.

How to Pass the OAE Biology Exam

What You Need to Know

  • Passing score: 220
  • Exam length: 150 questions
  • Time limit: 3h 15m appointment (3h testing)
  • Exam fee: $109

Keys to Passing

  • Complete 500+ practice questions
  • Score 80%+ consistently before scheduling
  • Focus on highest-weighted sections
  • Use our AI tutor for tough concepts

OAE Biology Study Tips from Top Performers

1Use the official Biology (007) framework as the primary outline until OAE fully launches Biology (706).
2Give extra time to genetics and evolution because it is the largest current domain at 27% of the assessment score.
3Practice data interpretation, controls, variables, safety, and evaluating evidence because Nature of Science is a full 20% domain.
4Connect cell structure to energy transformations, especially photosynthesis, respiration, membranes, and enzyme function.
5For organismal biology, study structure-function relationships across taxa and human organ systems rather than memorizing isolated facts.
6In ecology, practice interpreting population graphs, food webs, biogeochemical-cycle models, and human-impact scenarios.

Frequently Asked Questions

How many questions are on the OAE Biology exam?

The current OAE Biology (007) test page lists 150 multiple-choice questions. The page also notes that assessments may include questions being evaluated for future administrations that do not affect the score.

How long is the OAE Biology (007) appointment?

The official test page lists a 3 hour 15 minute total appointment, including 15 minutes for the tutorial and nondisclosure agreement and 3 hours of testing time. The same appointment length is listed for computer-based and online-proctored delivery.

What score do I need to pass OAE Biology?

The current passing score for OAE Biology (007) is 220. OAE says passing status is based on performance across the assessment.

How much does OAE Biology cost?

The official OAE Biology (007) test page lists an assessment fee of $109. Optional official preparation products, such as the practice assessment and Right Start subscriptions, are separate purchases when available.

Is OAE Biology changing in 2026?

Yes. The official assessments list shows Biology (007) with an anticipated testing end date in late 2026 and Biology (706) with an anticipated testing start date in late 2026. The 706 page says the revised assessment is pending State Board approval and that additional details will be posted as available.

Which OAE Biology domain carries the most weight?

In the active Biology (007) framework, Genetics and Evolution is the largest domain at 27% of the assessment score. Nature of Science, Biological Unity and Diversity, and Ecology and Environment are each 20%, while Biochemistry and Cell Biology is 13%.