1.2 Diagnostic Procedures & ABG Interpretation

Key Takeaways

  • ABG normal values: pH 7.35-7.45, PaCO2 35-45 mmHg, PaO2 80-100 mmHg, HCO3 22-26 mEq/L, SaO2 95-100%, base excess -2 to +2.
  • Use the fixed 5-step method: read pH, read PaCO2, read HCO3, name the primary disorder, then grade compensation.
  • The component whose abnormality matches the pH direction is the PRIMARY disorder; the opposing component shows compensation.
  • Expected acute compensation: PaCO2 changes ~10 mmHg shifts pH ~0.08, and acute respiratory changes move HCO3 ~1 mEq/L per 10 mmHg CO2.
  • PFT: FEV1/FVC below 70% defines obstruction; a reduced FVC with a normal ratio defines restriction (confirm with TLC).
  • Obstructive severity by FEV1 percent predicted: mild 60-79%, moderate 40-59%, severe under 40%.
  • A-a gradient = PAO2 minus PaO2; an elevated gradient localizes hypoxemia to the lung (shunt, V/Q mismatch, diffusion defect).
  • On chest film the endotracheal tube tip belongs 3-5 cm above the carina; too low produces right-mainstem intubation and left-lung collapse.
Last updated: June 2026

ABG Analysis — the Highest-Yield Skill

Arterial blood gas interpretation threads through nearly every TMC domain: oxygen titration, ventilator changes, and acid-base calls all hinge on it. Memorize the reference set, then apply one repeatable algorithm.

ParameterNormal RangeMeasures
pH7.35-7.45Net acid-base status
PaCO235-45 mmHgRespiratory (ventilation)
PaO280-100 mmHg (room air)Oxygenation
HCO322-26 mEq/LMetabolic (renal)
SaO295-100%Hemoglobin saturation
Base excess-2 to +2 mEq/LMetabolic indicator

The 5-Step Method

  1. pH — below 7.35 = acidemia, above 7.45 = alkalemia. A normal pH may still hide a fully compensated disorder.
  2. PaCO2 — above 45 = respiratory acidosis (hypoventilation); below 35 = respiratory alkalosis (hyperventilation).
  3. HCO3 — below 22 = metabolic acidosis; above 26 = metabolic alkalosis.
  4. Primary disorder — the component whose direction matches the pH is primary. pH 7.28 with PaCO2 58 = primary respiratory acidosis.
  5. Compensationuncompensated: one component abnormal, pH abnormal. Partially compensated: both abnormal, pH still abnormal. Fully compensated: both abnormal, pH back within 7.35-7.45.

A quick sanity rule: an acute 10 mmHg rise in PaCO2 lowers pH about 0.08; an acute 10 mmHg fall raises pH about 0.08. If the measured pH shift is far larger than CO2 alone predicts, a metabolic process is also present.

Pattern Recognition

DisorderpHPaCO2HCO3Classic Cause
Acute respiratory acidosisLowHighNormalOpioid overdose, neuromuscular failure
Chronic respiratory acidosisLow-normalHighHighCOPD, obesity hypoventilation
Respiratory alkalosisHighLowNormalAnxiety, pain, hypoxemia, PE, early sepsis
Metabolic acidosisLowNormal/lowLowDKA, lactic acidosis, renal failure
Metabolic alkalosisHighNormal/highHighVomiting, NG suction, diuretics
Combined acidosisVery lowHighLowCardiac arrest, severe shock

For metabolic acidosis, classify by anion gap = Na+ - (Cl- + HCO3-); normal is 8-12. A high gap (DKA, lactate, toxins) versus a normal gap (diarrhea, renal tubular acidosis) is a recurring exam distinction.

The A-a Gradient

The alveolar-arterial oxygen gradient localizes hypoxemia:

  • PAO2 = (FiO2 x (Pbar - 47)) - (PaCO2 / 0.8); at sea level Pbar - 47 = 713.
  • A-a gradient = PAO2 - PaO2; normal on room air is under 10-15 mmHg and widens with age (estimate age/4 + 4).
  • An elevated gradient means an intrapulmonary problem — shunt (ARDS, pneumonia), V/Q mismatch (PE, COPD), or diffusion impairment (fibrosis).
  • A normal gradient with hypoxemia points to hypoventilation or a low inspired FiO2 — the lung itself is transferring oxygen normally.

A practical companion is the PaO2/FiO2 ratio (P/F ratio), which grades oxygenation impairment without a calculation: a ratio at or below 300 with bilateral infiltrates meets the Berlin definition of acute respiratory distress syndrome, 200-300 is mild, 100-200 moderate, and below 100 severe ARDS. Because it needs only the ABG and the delivered FiO2, the P/F ratio is the number the exam expects you to quote when titrating PEEP and FiO2 on a ventilated patient.

Pulmonary Function Testing

PFTs classify chronic lung disease and appear on the TMC as result tables you must read at a glance.

MeasurementDefinitionNormal
FVCTotal volume forcibly exhaled after full inspiration>80% predicted
FEV1Volume exhaled in the first second>80% predicted
FEV1/FVCFraction of FVC exhaled in 1 second>0.70
PEFPeak expiratory flow>80% predicted
FEF25-75%Mid-expiratory flowSensitive early small-airway marker
DLCODiffusing capacity for carbon monoxideLow in emphysema/fibrosis, high in alveolar hemorrhage

Obstructive vs Restrictive

ParameterObstructiveRestrictive
FEV1/FVCDecreased (<0.70)Normal or increased
FVCNormal or decreasedDecreased (<80%)
TLCIncreased (air trapping)Decreased
RVIncreasedDecreased
ExamplesCOPD, asthma, bronchiectasis, CFFibrosis, kyphoscoliosis, obesity, neuromuscular

Obstruction is graded by FEV1 percent predicted — mild 60-79%, moderate 40-59%, severe <40%. A clinically important bronchodilator response is a 12% AND 200 mL improvement in FEV1 or FVC after a beta-agonist, which supports an asthma diagnosis.

Chest Radiograph Basics

Dense tissue is white (radiopaque); air is black (radiolucent).

FindingAppearanceConditions
ConsolidationWhite opacity, may show air bronchogramsPneumonia, pulmonary edema, ARDS
PneumothoraxBlack field, absent lung markingsSpontaneous, traumatic, barotrauma
Pleural effusionBasal white density, meniscus sign, blunted costophrenic angleCHF, infection, malignancy
HyperinflationFlattened diaphragms, increased AP diameterCOPD, severe asthma
AtelectasisVolume loss, trachea pulled TOWARD itMucus plug, postoperative
ET tubeTip 3-5 cm above carina (~aortic knob)Verify on every post-intubation film

A tube tip below the carina enters the right mainstem bronchus, ventilating only the right lung and collapsing the left — a classic stem in which the correct action is to withdraw the tube a few centimeters and recheck breath sounds.

Test Your Knowledge

ABG on room air: pH 7.52, PaCO2 28 mmHg, HCO3 24 mEq/L, PaO2 105 mmHg. The interpretation is:

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B
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D
Test Your Knowledge

PFT results: FEV1 45% predicted, FVC 82% predicted, FEV1/FVC 0.55. This pattern indicates:

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B
C
D
Test Your Knowledge

A hypoxemic patient has a widened A-a gradient. This finding most strongly suggests:

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B
C
D
Test Your Knowledge

On a post-intubation chest film the endotracheal tube tip should sit:

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B
C
D
Test Your Knowledge

ABG: pH 7.36, PaCO2 60 mmHg, HCO3 34 mEq/L. This represents:

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B
C
D