12.2 Endocrine Imaging (Thyroid, Parathyroid, Adrenal)

Key Takeaways

  • I-123 NaI is preferred for diagnostic thyroid morphology; Tc-99m pertechnetate shows trapping only (not organification); I-131 is used for uptake measurements and diagnostic/therapeutic whole-body pathways at very different activities
  • Thyroid cancer whole-body surveys use diagnostic I-123 or low-activity I-131 with TSH stimulation (thyroid hormone withdrawal or rhTSH), low-iodine diet, and strict separation from therapy-level I-131 doses
  • Parathyroid adenoma imaging classically uses Tc-99m sestamibi dual-phase washout and/or pertechnetate or I-123 subtraction; SPECT/SPECT-CT localizes ectopic or multigland disease
  • Adrenal/sympathetic tumor imaging uses I-123 MIBG (norepinephrine analog) with mandatory thyroid blockade and interfering-medication holds; NP-59 is a historical cholesterol-analog adrenocortical agent
  • High-yield traps: recent iodinated contrast or amiodarone suppressing uptake, skipped thyroid blockade for MIBG/free iodide, dual-phase sestamibi false positives from thyroid nodules, and confusing scan-dose vs therapy-dose radioiodine
Last updated: August 2026

12.2 Endocrine Imaging (Thyroid, Parathyroid, Adrenal)

Quick Answer: Thyroid: I-123 (preferred diagnostic imaging) vs Tc-99m pertechnetate (trapping only) vs I-131 (uptake / whole-body / therapy dose tiers). Parathyroid: sestamibi dual-phase ± subtraction, finish with SPECT/CT. Adrenal/sympathetic: I-123 MIBG + thyroid blockade; know NP-59 as legacy cortical imaging. Prep (iodine load, meds, TSH, blockade) makes or breaks the study.

Thyroid Imaging and Uptake

Physiology review

Follicular cells trap iodide (and pertechnetate) via the sodium-iodide symporter (NIS). Organification incorporates iodine into thyroglobulin/hormone pathways—I-123/I-131 participate; Tc-99m pertechnetate is trapped but not organified. Salivary glands and gastric mucosa also show NIS-related activity.

AgentWhat it showsTypical diagnostic use
I-123 NaITrapping + organification; excellent 159 keV imagingMorphology, nodules, uptake, preferred diagnostic scan
Tc-99m pertechnetateTrapping map onlyQuick thyroid morphology when iodine unavailable; discordant nodule workups
I-131 NaI (low activity)Uptake quantification; delayed whole-body capabilityUptake %; diagnostic whole-body cancer survey

Nodule language (exam favorites)

PatternTeaching meaning
Hot (hyperfunctioning) noduleSuppresses surrounding tissue; lower cancer probability but still manage clinically
Cold noduleDecreased function; higher concern—correlate ultrasound/FNA
Discordant noduleHot on pertechnetate, cold on radioiodine → failed organification concern

Uptake measurement

A neck probe or camera ROI measures percent uptake at protocol times (commonly 4–6 h and/or 24 h for iodine). Elevated uptake supports hyperthyroidism patterns (Graves, toxic nodule) when matched to labs; low uptake with thyrotoxicosis suggests thyroiditis or exogenous hormone. Recent iodinated IV contrast, amiodarone, iodine antiseptics, or high-iodine diet suppress measured uptake—document the exposure date.

Patient prep for diagnostic thyroid studies

Prep itemReason
Hold levothyroxine / liothyroxine or antithyroid drugs per orderAlters uptake and TSH drive
Avoid recent iodinated contrast (weeks–months effect)Floods iodine pool
Pregnancy/breastfeeding policiesAbsolute safety gates
NPO briefly for oral capsule only if protocol requiresConsistent absorption

Whole-Body Survey for Thyroid Metastases

After near-total thyroidectomy for differentiated thyroid cancer, diagnostic whole-body imaging maps remnant and iodine-avid mets (neck nodes, lungs, bone).

ElementTeaching standard
TracersI-123 whole-body or diagnostic I-131 (often ~1–5 mCi range—protocol/regulatory dependent)
TSH stimulationThyroid hormone withdrawal (hypothyroid TSH rise) or rhTSH injections
Low-iodine dietUsually ~1–2 weeks before dosing for surveys/therapy planning
Imaging timesI-123 often ~24 h; I-131 diagnostic may include 48–72 h views
Therapy I-131Much higher activity under written directive—never call a therapy dose a “scan dose”

Stunning (high-yield): larger diagnostic I-131 activities may reduce subsequent therapy uptake—many centers prefer I-123 surveys or minimize diagnostic I-131. Follow local protocol.

Traps: physiologic salivary/stomach/bowel/bladder activity; urine or saliva contamination; lactation breast uptake—survey gown/skin for unexpected foci.

Parathyroid Imaging

Primary hyperparathyroidism workups seek a hyperfunctioning adenoma (less often hyperplasia or carcinoma) before minimally invasive surgery.

Dual-phase sestamibi

Tc-99m sestamibi is taken up by both thyroid and abnormal parathyroid tissue (mitochondria-rich adenomas). Early images (~10–15 min) show thyroid + parathyroid; delayed images (~1.5–2.5 h) rely on faster thyroid washout so adenoma persists (“differential washout”).

MethodIdea
Dual-phase sestamibiEarly vs delayed persistence at adenoma site
SubtractionSestamibi minus Tc-99m pertechnetate or I-123 thyroid map isolates parathyroid focus
SPECT / SPECT-CTDepth, ectopic mediastinal glands, multigland disease, anatomic roadmap

Typical adult activity is often ~20–25 mCi sestamibi IV (lab-specific). SPECT/CT is preferred when available for surgical localization.

Traps: thyroid nodules can retain sestamibi (false positive); small hyperplastic glands may be missed (false negative); recent IV contrast CT is complementary but not a substitute for functional localization. Always correlate calcium, PTH, and ultrasound.

Adrenal Imaging Concepts

I-123 MIBG (iobenguane)

MIBG is a norepinephrine analog taken up by the norepinephrine transporter into adrenergic tissue—pheochromocytoma, paraganglioma, neuroblastoma, and related tumors. Thyroid blockade (SSKI, Lugol’s, or perchlorate per protocol) is mandatory. Hold interfering drugs (selected antidepressants, labetalol, sympathomimetics, cocaine, others per insert) to avoid false-negative studies. Imaging typically includes 24-hour planar ± SPECT/CT; adult activity often ~10 mCi IV order of magnitude.

NP-59 (historical adrenocortical agent)

I-131 NP-59 (iodomethylnorcholesterol) is a cholesterol analog historically used to image the adrenal cortex (e.g., adenoma vs hyperplasia patterns in hyperaldosteronism/Cushing workups) with multi-day imaging and dexamethasone suppression variants. Modern practice often favors CT/MRI and venous sampling, but CNMT items may still expect: cortical cholesterol pathway, multi-day imaging, and dexamethasone suppression protocol concept. Do not confuse NP-59 (cortex) with MIBG (medulla/sympathetic).

PathwayAgent classTarget
Adrenal medulla / sympathetic tumorsMIBGNET uptake storage granules
Adrenal cortex (legacy)NP-59Cholesterol analog uptake

Endocrine Prep and Trap Matrix

StudyMust-do prepClassic failure mode
Thyroid uptake/scanNo recent iodine load; correct med holdsFalsely low uptake after contrast/amiodarone
Cancer whole-body I-123/I-131TSH stimulation + low-iodine dietResidual diet iodine → poor lesion visualization
Parathyroid sestamibiEarly + delayed ± SPECT/CT; know thyroid diseaseHot thyroid nodule called “adenoma”
MIBGThyroid blockade + med reconciliationFree iodide in thyroid; drug-related false negative

Selection cheat sheet: morphology with low dose → I-123; trapping-only quick look → pertechnetate; iodine-avid cancer map → stimulated whole-body iodine; PTH adenoma localization → sestamibi SPECT/CT; pheo/neuroblastoma → MIBG.

Test Your Knowledge

Why might a technologist prefer I-123 over Tc-99m pertechnetate when evaluating a thyroid nodule’s organification behavior?

A
B
C
D
Test Your Knowledge

Which preparation package is most appropriate before a diagnostic radioiodine whole-body survey for differentiated thyroid cancer?

A
B
C
D
Test Your Knowledge

In dual-phase Tc-99m sestamibi parathyroid imaging, what is the expected behavior of a typical parathyroid adenoma relative to normal thyroid tissue?

A
B
C
D