13.2 Cardiac Function, Viability, and Special Studies

Key Takeaways

  • First-pass radionuclide angiography measures right and left ventricular EF and wall motion from a tight IV bolus; MUGA (ERNA) uses labeled RBCs for high-precision LVEF at rest (± stress) and SPECT blood-pool options
  • Cardiac shunt quantification compares systemic vs pulmonary flow using first-pass curves when ordered
  • Tc-99m PYP planar + SPECT for ATTR cardiac amyloidosis: image ~1 h and/or 3 h; semi-quantitative visual grades and H/CL (heart-to-contralateral lung) ratio concepts support interpretation
  • Cardiac sarcoidosis evaluation favors FDG PET with strict dietary/prep protocols; I-123 MIBG assesses cardiac sympathetic innervation
  • Viability: Tl-201 rest–redistribution (or stress-redistribution-reinjection pathways) vs FDG PET metabolic imaging after glucose loading/insulin euglycemia prep
Last updated: August 2026

13.2 Cardiac Function, Viability, and Special Studies

Quick Answer: First-pass = bolus EF/wall motion (RV & LV). MUGA/ERNA = Tc-99m labeled RBCs, high-precision serial LVEF. Shunt studies use first-pass curve analysis. PYP = ATTR amyloid (1 h / 3 h, visual grade, H/CL). FDG PET = sarcoid (prep!) and viability. MIBG = innervation. Tl-201 rest-redistribution or FDG for viability when revascularization is considered.

Order these studies for function, infiltrate/inflammation, innervation, or hibernating myocardium—not routine CAD screening alone.

First-Pass Radionuclide Angiography

A compact IV bolus (often Tc-99m DTPA, pertechnetate, or lab-chosen first-pass agent) is imaged with rapid frames as activity traverses SVC → RV → lungs → LV → aorta.

OutputTeaching value
RVEF / LVEFCount-based ejection fractions from time-activity curves
Wall motionRegional qualitative assessment during transit
Shunt cluesAbnormal recirculation patterns

Tech keys: large vein, bolus integrity (no infiltrate, rapid flush), high frame rate, correct ROIs. A fragmented bolus invalidates quantitative EF.

Gated Blood Pool (MUGA / ERNA)

Equilibrium radionuclide angiocardiography (ERNA/MUGA) uses Tc-99m labeled autologous RBCs (in vivo, modified in vivo, or in vitro). After mixing, ECG-gated planar images (classic best septal LAO for EF) sum many beats into one representative cycle.

SettingNotes
Rest MUGASerial LVEF (e.g., chemotherapy cardiotoxicity)
Stress MUGAExercise or pharmacologic stress with gating
SPECT blood poolTomographic volumes/wall motion
Typical activityOften ~20–30 mCi Tc-99m RBCs

Why MUGA endures: excellent LVEF reproducibility when labeling and ROI methods stay consistent. QC includes labeling efficiency, arrhythmia rejection, count density, and background ROI. Highly irregular AF degrades frames—document and apply lab filters.

Labeling pitfalls matter: poor RBC tag leaves free Tc-99m (stomach, thyroid, soft tissue) and undercuts chamber contrast. Verify the labeling method on the order (in vitro kits often give higher efficiency) and image only after adequate equilibration.

Cardiac Shunt Evaluation

Left-to-right shunts alter pulmonary transit on first-pass curves; quantitative methods estimate Qp/Qs. Right-to-left shunts may use Tc-99m MAA (systemic foci after venous injection). Match agent and analysis to the ordered shunt direction.

Cardiac Amyloidosis: Tc-99m PYP

ATTR cardiac amyloid is often Tc-99m PYP-avid; AL is less so—labs combine imaging with monoclonal protein workups. Technologists still must execute timing and SPECT correctly.

Protocol elementTeaching practice
ActivityOften ~10–25 mCi Tc-99m PYP IV
Imaging times~1 hour and/or ~3 hours planar + SPECT/SPECT-CT
Visual gradingHeart vs rib (0–3 style; grade ≥2 at 3 h supports ATTR pattern when criteria met)
H/CL ratioHeart / contralateral lung counts; elevated ratio (classic teaching near ~1.5 at 1 h—use lab cutoffs) supports abnormal retention
SPECT roleConfirms myocardial, not blood-pool, uptake

Note recent MI (classic infarct-avid use of PYP) and keep ROIs consistent.

Planar-only reading can mistake blood-pool activity for myocardial uptake—SPECT (or SPECT/CT) is required to localize tracer to myocardium. Time both 1 h and 3 h sets the same way each visit so visual grades and H/CL ratios remain comparable.

Cardiac Sarcoidosis PET

F-18 FDG PET/CT (± perfusion imaging) detects active myocardial inflammation. Prep is strict and lab-specific: prolonged high-fat, low-carbohydrate diet, fasting, sometimes heparin, to suppress normal myocyte glucose uptake. Failed prep → diffuse physiologic FDG that mimics or masks disease. This is not a casual oncology FDG instruction set.

Cardiac MIBG

I-123 MIBG maps sympathetic innervation (heart-to-mediastinum ratios, washout). Hold interfering drugs per lab list; follow iodine thyroid-protection rules when applicable. MIBG is not a substitute for MPI ischemia mapping.

Myocardial Viability

ApproachConceptWhen chosen
Tl-201 rest–redistributionRest uptake + 3–4 h (sometimes 24 h) fill-in = viable hibernationSPECT viability without FDG
Tl-201 stress–redistribution ± reinjectionStress defect with later uptakeCombined ischemia + viability
F-18 FDG viability PETFDG-avid tissue in a perfusion defect = mismatch (hibernating); matched severe reduction = scarMetabolic gold standard when available
Tc-99m rest uptake (supportive)Preserved rest perfusion favors viabilityAdjunct, less definitive

FDG viability prep uses glucose loading/insulin euglycemia to stimulate myocyte FDG uptake—the opposite goal of sarcoid suppression prep. Swapping those preps is a classic board trap.

When reporting viability language at the tech level, remember: perfusion-metabolism mismatch suggests hibernating myocardium that may improve after revascularization; matched severe reduction suggests scar with less recovery potential. Actual revascularization decisions remain clinical.

Choosing the Study

Clinical questionNuclear package
Serial LVEF on cardiotoxic chemoRest MUGA
RV function / shunt / bolus EFFirst-pass
ATTR amyloidTc-99m PYP 1 h/3 h + SPECT, H/CL
Cardiac sarcoid inflammationFDG PET with suppression prep
Sympathetic denervationI-123 MIBG
Hibernation before revascularizationTl-201 redistribution and/or FDG viability
Daily CAD ischemia vs scarMPI (± gating) first (§13.1)

Bottom line: name the question, pick the agent and prep, and defend bolus quality, labeling, timing, and ratios.

Test Your Knowledge

A patient needs highly reproducible serial LVEF measurements during anthracycline chemotherapy. Which study is the classic nuclear medicine choice?

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Test Your Knowledge

For Tc-99m PYP imaging of suspected ATTR cardiac amyloidosis, which technical/interpretive package is most appropriate?

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D
Test Your Knowledge

How does patient preparation for F-18 FDG cardiac sarcoidosis PET differ in purpose from preparation for FDG myocardial viability PET?

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