13.1 Myocardial Perfusion Imaging

Key Takeaways

  • MPI maps relative myocardial blood flow with Tc-99m sestamibi/tetrofosmin SPECT (± ECG gating, prone, SPECT/CT attenuation) or PET agents Rb-82 and N-13 ammonia
  • One-day Tc protocols use low rest then high stress (≈3× activity); two-day uses roughly equal high doses; inject at peak exercise or peak pharmacologic hyperemia
  • Patient prep: NPO as ordered, hold caffeine 12–24 h for vasodilator stress, hold beta blockers for exercise stress when the order requires them
  • Common artifacts—breast attenuation (anterior/anteroseptal), diaphragmatic (inferior), motion, and LBBB-related septal defects—must be recognized before calling ischemia or scar
  • Gated SPECT adds wall motion and EF; fixed defect with normal thickening often favors attenuation over true infarct
Last updated: August 2026

13.1 Myocardial Perfusion Imaging

Quick Answer: MPI images relative coronary flow at stress and rest. Tc-99m sestamibi/tetrofosmin SPECT (± gating, prone, SPECT/CT AC) is standard; PET uses Rb-82 or N-13 ammonia. Sequence and dose ratios (one-day low→high vs two-day equal) protect image quality. Prep = NPO, no caffeine 12–24 h for vasodilators, hold β-blockers for exercise when ordered. Flag breast, diaphragm, motion, LBBB artifacts.

Domain V cardiac items test protocol choice, timing, prep, and tech-level artifact recognition—not full cardiologist scoring of every coronary territory.

Clinical Role of MPI

IndicationTeaching point
Suspected CAD / chest painStress–rest patterns support ischemia vs scar
Known CAD risk stratificationExtent/severity of ischemia guides management
Preoperative cardiac riskFunctional capacity plus perfusion when indicated
Viability adjunct (selected)Rest uptake / PET pathways (see §13.2)

The technologist owns dose, injection timing, positioning, gating, and raw-data QC.

Correlate symptoms, ECG changes, and achieved heart rate or pharmacologic adequacy with the images. A technically perfect scan after a failed stress still underestimates ischemia—document peak HR, symptoms, ST changes, and whether caffeine or β-blockers were held.

Planar vs SPECT MPI

Early MPI used planar multi-view imaging (anterior, LAO, left lateral). Planar is historical foundation and rare fallback; modern practice is SPECT (and hybrid SPECT/CT or PET/CT). Tomography separates overlapping walls that planar cannot.

SPECT MPI: Gated and Non-Gated

After IV injection, wait for blood-pool and hepatobiliary clearance (often ~30–60 min rest, ~15–45 min post-stress; tetrofosmin often earlier than sestamibi). Acquire SPECT with arms up when tolerated.

ModeWhat you getWhy it matters
Non-gated SPECTPerfusion onlyUse if irregular rhythm prevents gating
ECG-gated SPECTPerfusion + wall motion, thickening, LVEFNormal motion favors attenuation over infarct
Supine SPECTDefault geometryBreast and diaphragm attenuation common
Prone SPECTBelly downOften reduces inferior diaphragmatic attenuation
SPECT/CTSPECT + low-dose CT attenuation correction (AC)Still review non-AC and raw projections

Prone + supine (or AC + non-AC) comparison helps when fixed inferior or anterior defects look artifactual. CT-AC caveats: misregistration from breathing or position change creates false defects—check fused alignment before trusting AC-only slices.

PET Myocardial Perfusion

AgentHalf-life / sourceTeaching notes
Rb-82 chloride75 s; Sr-82 generatorRest and stress same session; absolute flow possible
N-13 ammonia10 min; cyclotronHigh extraction; needs nearby cyclotron
O-15 water (selected)Very short; cyclotronQuantitation/research

PET offers higher resolution and routine CT attenuation correction. Stress is usually pharmacologic because of short half-lives. Inject and image on a tight clock—especially Rb-82.

Tc-99m Protocols: One-Day vs Two-Day

Tc-99m agents show minimal clinical redistribution; separate stress and rest injections are required.

ProtocolDose pattern (adult order-of-magnitude)Sequencing logic
One-day rest → stressRest ~8–12 mCi, stress ~24–36 mCi (≈3:1)High second dose dominates residual
One-day stress → rest (selected)Stress first then higher rest, or stress-only if normalLab-specific triage pathways
Two-day~20–30 mCi each day (roughly equal)Large patients; avoid same-day residual
Stress-only (selected low-risk)Single stress ~25–40 mCi classRest canceled if stress clearly normal

Injection timing: at peak treadmill exercise (continue ~1 min when safe) or at peak pharmacologic hyperemia (during adenosine, after regadenoson bolus, or timed with dipyridamole). Secure IV—extravasation ruins relative counts.

Same-day dual-isotope protocols (historical Tl-201 rest + Tc-99m stress) appear on older exams but are less common now; know that different energies and redistribution properties change processing. For pure Tc one-day work, never invert the high/low ratio without a written protocol reason.

Patient Preparation (High-Yield)

Prep itemWhy
NPO / light meal as orderedLower GI activity; safer stress
Hold caffeine 12–24 h before adenosine, dipyridamole, regadenosonCaffeine antagonizes A2A hyperemia → false-negative MPI
Hold β-blockers for exercise when orderedProtects diagnostic heart-rate response
Other meds per orderDocument what the patient took
History: LBBB, paced rhythm, COPD/asthma, caffeine todayChooses modality; predicts artifacts
Pregnancy screen; IV; coach stillnessSafety and motion control

Common Artifacts and Pitfalls

ArtifactTypical appearanceTech response
Breast attenuationFixed anterior/anterolateralRotating cine; prone or AC; normal gated motion
Diaphragmatic attenuationFixed inferior wallProne, AC, gated thickening
MotionCine discontinuities, false defectsRepeat; validated motion correction
LBBB / RV paced rhythmSeptal changes ± “reversible” lookPrefer pharmacologic stress when ordered; document rhythm
Subdiaphragmatic liver/bowelInferior scatter / normalization errorDelay, hydrate, fatty snack, re-image

Review the rotating projection cine on every study before reconstruction sign-off. Truncation, upward creep, and arm-down positioning create defects that look like CAD until the raw data are inspected.

Bottom line: match agent and camera mode to the question, protect dose ratio and injection timing, enforce caffeine and med holds, and review raw projections + gating before calling ischemia or scar.

Test Your Knowledge

A one-day rest–stress Tc-99m sestamibi protocol is ordered. Which dose relationship best matches standard teaching practice?

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Test Your Knowledge

Why must patients avoid caffeine for 12–24 hours before pharmacologic vasodilator MPI with adenosine, dipyridamole, or regadenoson?

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D
Test Your Knowledge

Which statement best describes a common inferior-wall fixed defect on supine SPECT MPI in a male patient with normal inferior wall motion on gated images?

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D