Key Takeaways

  • ATP-PC system provides immediate energy (0-10 seconds) without oxygen; limited by phosphocreatine stores.
  • Glycolysis breaks down glucose to produce ATP; fast glycolysis produces lactate, slow glycolysis feeds the oxidative system.
  • The oxidative system produces the most ATP per molecule but is the slowest; dominant during low-intensity, long-duration activities.
  • EPOC (excess post-exercise oxygen consumption) represents elevated metabolism after exercise for recovery processes.
  • Substrate utilization shifts from primarily fat at low intensities to primarily carbohydrates at high intensities.
Last updated: January 2026

Bioenergetics and Metabolism

Quick Answer: Three energy systems produce ATP: the phosphagen system (immediate, 0-10 sec), glycolysis (short-term, 10 sec-2 min), and oxidative system (long-term, >2 min). All systems are always active, but their relative contribution depends on exercise intensity and duration. High-intensity activities rely more on anaerobic systems; low-intensity activities rely more on the oxidative system.

ATP: The Energy Currency

Adenosine triphosphate (ATP) is the only energy source directly used for muscle contraction:

  • Stored ATP in muscle is very limited (~80-100g total body stores)
  • Enough for only 1-2 seconds of maximal exercise
  • Must be continuously regenerated from ADP (adenosine diphosphate)

ATP → ADP + Pi + Energy (for muscle contraction)

The three energy systems regenerate ATP at different rates and capacities.

The Three Energy Systems

SystemPrimary FuelRate of ATP ProductionATP YieldDurationOxygen Required
Phosphagen (ATP-PC)Creatine phosphateFastestLimited0-10 secondsNo (anaerobic)
GlycolyticGlucose/GlycogenFastModerate10 sec-2 minNo (anaerobic)
OxidativeCarbs, Fats, ProteinSlowestHighest>2 minutesYes (aerobic)

1. Phosphagen System (ATP-PC System)

The phosphagen system provides immediate energy for high-intensity, short-duration activities.

Key Characteristics

FactorDescription
SubstratesATP and creatine phosphate (CP) stored in muscle
Duration0-10 seconds of maximal effort
ATP production rateFastest of all systems
Limiting factorDepletion of CP stores
Recovery50% in ~30 sec; full recovery in 3-5 minutes

The Reaction

Creatine Phosphate + ADP → ATP + Creatine (via creatine kinase)

Applications

ActivityDurationPhosphagen Contribution
40-yard dash~5 secVery high
Vertical jump<1 secPrimary
1RM attempt~3 secVery high
Baseball swing<1 secPrimary

Exam Tip: The phosphagen system is the dominant energy system for single maximal efforts lasting less than 10 seconds. Rest intervals of 2-5 minutes are needed for complete CP recovery between sets.

Creatine Supplementation

  • Increases intramuscular creatine and phosphocreatine stores
  • Improves performance in repeated high-intensity bouts
  • Loading dose: 20g/day for 5-7 days, then 3-5g/day maintenance
  • One of the most researched and effective legal supplements

2. Glycolytic System

The glycolytic system breaks down glucose (or glycogen) to produce ATP through a series of chemical reactions.

Two Forms of Glycolysis

TypeEnd ProductOxygenATP YieldSpeed
Fast (anaerobic) glycolysisLactate (lactic acid)Not required2 ATP per glucoseFast
Slow (aerobic) glycolysisPyruvate → enters oxidative systemAvailable2 ATP + more via oxidationSlower

Fast Glycolysis Pathway

Glucose → Pyruvate → Lactate + 2 ATP (net)

StepDetails
Input1 glucose molecule (or 1 glycogen unit)
ATP cost2 ATP invested (1 if from glycogen)
ATP produced4 ATP
Net ATP2 ATP (3 if from glycogen)
End productLactate (when oxygen insufficient)

Lactate: Not the Villain

Modern understanding of lactate:

Old ViewCurrent Understanding
"Waste product"Valuable metabolic intermediate
Causes muscle sorenessDOMS is from muscle damage, not lactate
Limits performanceImportant fuel source for heart, brain, and muscles
Should be avoidedLactate clearance is a trainable adaptation

Key Point: Lactate can be converted back to glucose in the liver (Cori cycle) or used directly as fuel by the heart and other tissues.

Lactate Threshold

  • The intensity at which lactate production exceeds clearance
  • Typically occurs at 50-60% VO2max in untrained individuals
  • Can improve to 70-80% VO2max with training
  • Important marker for endurance performance

3. Oxidative (Aerobic) System

The oxidative system produces ATP through aerobic metabolism in the mitochondria.

Components

  1. Aerobic glycolysis: Glucose → Pyruvate → Acetyl-CoA
  2. Krebs cycle (Citric Acid Cycle): Acetyl-CoA → CO2 + electron carriers
  3. Electron Transport Chain (ETC): Electron carriers → ATP + H2O

ATP Yield from Different Substrates

SubstrateATP Yield (approximately)
1 glucose molecule30-32 ATP
1 palmitate (fatty acid)106 ATP
1 amino acidVaries (typically 15-20 ATP)

Fat Oxidation (Beta-Oxidation)

Fats provide the highest ATP yield per molecule but require:

  • More oxygen per ATP produced
  • More time to break down
  • Lower intensity exercise for optimal use
FactorCarbohydrateFat
ATP per O2More efficientLess efficient
Total ATP yield30-32 per glucose106+ per fatty acid
Speed of availabilityFastSlow
Optimal intensityHighLow to moderate

The Crossover Concept

As exercise intensity increases, the body shifts from fat to carbohydrate oxidation:

IntensityPrimary Fuel
Rest~60% fat, ~40% carbs
25% VO2max~70% fat, ~30% carbs
50% VO2max~50% fat, ~50% carbs
65% VO2max~40% fat, ~60% carbs
85% VO2max~15% fat, ~85% carbs
Maximal~100% carbs

Exam Tip: Fat oxidation requires oxygen and is maximized at low to moderate intensities (around 60-65% VO2max). High-intensity exercise relies primarily on carbohydrate oxidation.

Energy System Contributions by Activity

ActivityPhosphagenGlycolyticOxidative
Shot put90%10%0%
100m sprint50%45%5%
400m sprint25%65%10%
800m run10%50%40%
1500m run5%25%70%
Marathon0%5%95%

EPOC: Excess Post-Exercise Oxygen Consumption

EPOC (formerly called "oxygen debt") is the elevated oxygen consumption that occurs after exercise:

Causes of EPOC

Fast Component (minutes)Slow Component (hours)
Replenish muscle ATPElevated body temperature
Replenish creatine phosphateElevated heart rate and breathing
Reload muscle myoglobin with O2Tissue repair
Reload hemoglobin with O2Hormone effects (catecholamines)
Lactate conversion

EPOC Magnitude Factors

FactorEffect on EPOC
Exercise intensityHigher intensity = greater EPOC
Exercise durationLonger duration = greater EPOC
Type of exerciseResistance training > steady-state cardio
Training statusTrained individuals have faster recovery

Exam Tip: EPOC is greater following high-intensity interval training (HIIT) and resistance training compared to steady-state aerobic exercise at the same total caloric expenditure.

Substrate Utilization Summary

Factors Favoring Fat Oxidation

  • Low exercise intensity
  • Longer exercise duration (after glycogen depletion begins)
  • Fasted state
  • Endurance training adaptations
  • Higher aerobic capacity

Factors Favoring Carbohydrate Oxidation

  • High exercise intensity
  • Short duration, high-power activities
  • Fed state (high carbohydrate availability)
  • Untrained state
  • Type II fiber recruitment
Test Your Knowledge

A football lineman performs a single maximal effort lasting approximately 5 seconds. Which energy system is the PRIMARY contributor?

A
B
C
D
Test Your Knowledge

Which energy system produces the MOST ATP per molecule of substrate?

A
B
C
D
Test Your Knowledge

At what exercise intensity is fat oxidation typically MAXIMIZED?

A
B
C
D
Test Your Knowledge

What is the approximate time needed for full phosphocreatine (PC) recovery after a maximal effort?

A
B
C
D