1.2 Principles of Infection Control & Decontamination Levels

Key Takeaways

  • Infection control in the salon runs across three tiers — cleaning (sanitation), disinfection, and sterilization — and cleaning must mechanically remove all visible soil and organic residue before any chemical step.
  • Chemical disinfection destroys vegetative pathogenic bacteria, viruses, and fungi on nonporous surfaces, but it does not destroy bacterial endospores.
  • Under CDC Standard Precautions, cosmetologists must treat all human blood, body fluids, secretions (except sweat), and non-intact skin as potentially infectious.
  • Multi-use implements constructed of nonporous materials can be cleaned and disinfected, whereas porous single-use tools must be discarded immediately after a single service.
  • Section 1175.115(b)(14) requires thorough hand washing before and after serving each client, and (b)(15) requires the licensee to ensure the client's own hands or feet are washed before any manicure or pedicure.
Last updated: September 2026

1.2 Principles of Infection Control & Decontamination Levels

Infection control is the discipline of eliminating or significantly reducing the transmission of infectious pathogens between clients, practitioners, and salon work environments. To safeguard public health and comply with regulations established by the Occupational Safety and Health Administration (OSHA) and the Illinois Department of Financial and Professional Regulation (IDFPR), beauty professionals must operate under a rigorous decontamination hierarchy. Decontamination is defined as the physical or chemical removal of pathogens from an object or surface, rendering it safe for human handling.


The Three Levels of Decontamination

Decontamination is structured into three progressive, hierarchical levels: cleaning (sanitation), disinfection, and sterilization.

+-----------------------------------------------------------------------------------------+
|                               DECONTAMINATION HIERARCHY                                 |
+---------------+-------------------------+---------------------+-------------------------+
| Level         | Process & Mechanism     | Target Organisms    | Salon Application       |
+---------------+-------------------------+---------------------+-------------------------+
| 1. Cleaning   | Mechanical friction,    | Removes dirt, oils, | Counters, shampoo bowls,|
| (Sanitation)  | soap/detergent & water  | gross debris, drops | pre-cleaning for tools  |
|               |                         | microbial load      | (MANDATORY step 1)      |
+---------------+-------------------------+---------------------+-------------------------+
| 2. Disinfection| Chemical agents        | Destroys bacteria,  | Multi-use shears, combs,|
|               | (EPA-registered liquids,| viruses, and fungi; | nippers, workstations,  |
|               | sprays, wipes)          | DOES NOT kill spores| pedicure basins         |
+---------------+-------------------------+---------------------+-------------------------+
| 3. Steril-    | Pressurized steam       | Kills ALL microbial | Medical devices, lancets|
|    ization    | (autoclave 121°C/15psi) | life, including     | that puncture skin;     |
|               | or dry heat             | bacterial endospores| spore testing verified  |
+---------------+-------------------------+---------------------+-------------------------+

Level 1: Cleaning (Sanitation)

Cleaning (historically referred to as sanitation) is the mechanical removal of visible surface dirt, debris, hair clippings, dead skin cells, sebum, and organic residues through the application of soap or detergent, warm water, and physical friction (such as scrubbing with a firm, clean nylon brush).

  • Mechanism: Cleaning physically detaches soil and reduces the overall microbial population on a surface, but it does not chemically kill all pathogenic microorganisms or destroy bacterial endospores.
  • Mandatory Prerequisite: Cleaning is the non-negotiable first step of all infection control protocols. If an implement or styling station is not thoroughly cleaned first, residual organic matter (e.g., skin flakes, hair styling waxes, blood droplets) creates a protective physical barrier that shields microorganisms from chemical contact, while proteins can chemically bind to and neutralize active disinfectant agents.
  • Sanitizer vs. Disinfectant: In industry terminology, chemical hand sanitizers (e.g., 60–70% ethyl alcohol gels) are intended strictly for cleaning living skin when water and soap are temporarily unavailable. Hand sanitizers must never be used to decontaminate salon tools or hard surfaces.

Level 2: Disinfection

Disinfection is the chemical process that destroys virtually all recognized pathogenic microorganisms—including vegetative bacteria, pathogenic fungi, and bloodborne viruses—on nonporous hard surfaces.

  • Limitations: Chemical disinfection does not kill bacterial endospores. Because true endospores are rarely encountered on intact skin surfaces during standard cosmetology services, EPA-registered chemical disinfection represents the universally mandated industry standard for multi-use implements, styling stations, shampoo bowls, and treatment tables.
  • Immersion Standards: To achieve full disinfection, multi-use implements must remain completely submerged in an EPA-registered liquid disinfectant for the manufacturer's specified duration—typically a minimum of 10 minutes.

Level 3: Sterilization

Sterilization represents the highest, most complete level of decontamination achievable. It is defined as the total destruction and eradication of all microbial life, including vegetative bacteria, viruses, fungi, and highly resistant bacterial endospores.

  • Methods: Sterilization requires physical agents capable of penetrating microbial cell walls, most commonly saturated steam under high pressure inside an autoclave, dry heat ovens, or specialized toxic chemical gas chambers (ethylene oxide).
  • Cosmetology Relevance: General cosmetology services deal with intact skin and keratinized structures; therefore, routine sterilization of styling tools is not required by Illinois law. However, any implement intended to puncture the skin barrier or penetrate vascular tissue (such as dermal extraction lancets or microdermabrasion tips in advanced aesthetics) is categorized as a critical item and must undergo full steam autoclave sterilization between clients.

CDC Guidelines & Standard Precautions

Cosmetologists operate under safety guidelines established by the Centers for Disease Control and Prevention (CDC) and OSHA.

Evolution to Standard Precautions

In 1985, in response to the emerging HIV/AIDS epidemic, the CDC introduced Universal Precautions, an infection control concept asserting that all human blood and specified bodily fluids containing visible blood must be treated as infectious for bloodborne pathogens.

In 1996, the CDC expanded this model into Standard Precautions, which represent the current legal and clinical standard across all healthcare and personal care professions:

  • Core Rule: Cosmetologists must assume that all human blood, all body fluids, secretions, excretions (except sweat), non-intact skin, and mucous membranes are potentially infectious with HIV, Hepatitis B, Hepatitis C, and other communicable pathogens, regardless of the client's apparent health, background, or social status.
  • Sweat Exemption: Sweat is the only bodily fluid that does not transmit bloodborne pathogens, provided it is not visibly contaminated with blood.

Hand Hygiene Protocols

Handwashing is universally recognized as the single most effective barrier against pathogen cross-contamination:

  1. Wet hands with warm running water and apply liquid antimicrobial or plain liquid soap from a closed dispenser (bar soaps collect bacterial slime and are prohibited).
  2. Rub hands together vigorously for a minimum of 20 seconds, working lather across the palms, backs of hands, wrists, interdigital spaces (between fingers), and beneath the free edges of fingernails using a clean nail brush.
  3. Rinse thoroughly with warm water flowing downward from wrists to fingertips.
  4. Dry hands completely using a clean, disposable single-use paper towel. Common cloth towels or roll towels are strictly prohibited in Illinois salons because they retain dampness and harbor fungal spores and bacterial colonies.
  5. Use the paper towel to turn off the faucet handles to avoid recontaminating clean hands.

When Illinois requires it. Section 1175.115(b)(14) of Title 68 is explicit: licensees shall observe and follow thorough hand washing with soap and water, an equally effective cleansing solution, or a waterless hand sanitizer before and after serving each client — not merely after a visibly dirty service. Subsection (b)(15) adds a second, frequently missed duty: the licensee must ensure that each client's own hands or feet are washed with soap and water or a waterless hand sanitizer before any manicuring or pedicuring service begins.

Personal Hygiene & Professional Image

"Hygiene" is a named competency in the Salon Safety and Sanitation area of the Illinois content outline, and it means the practitioner's own condition, not just the tools. In a profession that works within inches of a client's face, scalp, and hands for an hour at a time, personal hygiene is an infection-control control point and a client-retention factor at the same time.

Hygiene DomainProfessional StandardWhy the Exam Cares
Daily body cleanlinessDaily bath or shower, effective antiperspirant or deodorant, no heavy fragranceBody odor and heavy scent both drive client complaints; some clients react to fragrance during chemical services
Oral hygieneTeeth brushed, breath managed, no smoking odor on breath, hands, or clothingThe operator's face is routinely within 12 to 18 inches of the client's face during consultation and haircutting
Hands and nailsNails short, smooth, and clean; no chipped enhancements; cuts covered with a waterproof dressing and glovesRough or damaged nails scratch a client's scalp; broken skin on the operator is a two-way pathogen route
HairThe operator's own hair clean, styled, and secured back off the faceIt is the profession's most visible advertisement, and loose hair falls into open product containers
AttireClean, laundered clothing daily; closed-toe, non-slip, low-heel shoes; a fresh apron or smock for chemical workClosed toes protect against dropped shears and spilled chemicals; a changed smock stops chemical residue traveling client to client
JewelryMinimal; remove rings, bracelets, and long dangling pieces before servicesJewelry harbors microorganisms, snags hair, and prevents thorough hand washing
Own healthDo not work while contagiousSection 1175.115(b)(33) bars an owner or manager from knowingly permitting a person with a serious communicable disease to work on the premises, and (b)(35) bars a licensee from working on a client who has one

Two hygiene habits that fail inspections. First, working with an uncovered cut or hangnail on your own hand — an open lesion on the operator is a bloodborne-pathogen exposure route in both directions and must be covered and gloved. Second, eating or drinking at the station: food residue on hands defeats the pre-client hand wash, and open containers in the service area invite contamination of products nearby.

Personal Protective Equipment (PPE)

Cosmetologists must don appropriate PPE based on occupational hazard exposure:

  • Gloves: Single-use, disposable nitrile or vinyl gloves should be worn whenever there is a risk of contact with blood, body fluids, open lesions, or concentrated chemical disinfectants. (Nitrile is preferred over latex to prevent allergic sensitization in clients and staff).
  • Eye Protection: Safety glasses or chemical splash goggles must be worn during chemical disinfectant measuring, mixing, and pouring.

Hospital-Grade vs. Tuberculocidal Disinfectants

The Environmental Protection Agency (EPA) registers and regulates chemical antimicrobials sold in the United States. Salons must use disinfectants that possess an official EPA registration number on the label.

Disinfectant TypeIndicator Organisms DestroyedSalon Application
Hospital-Grade DisinfectantStaphylococcus aureus, Salmonella enterica, Pseudomonas aeruginosaGeneral nonporous salon surfaces, styling stations, multi-use shears, combs, and brushes
Tuberculocidal DisinfectantMycobacterium tuberculosis (plus hospital-grade targets)Tools or surfaces subjected to blood spills, body fluids, or puncture contamination
  • Hospital-Grade Disinfectants: To obtain an EPA hospital registration, a chemical disinfectant must demonstrate proven bactericidal activity against three specific target organisms: Staphylococcus aureus (gram-positive cocci), Salmonella enterica (gram-negative intestinal bacilli), and Pseudomonas aeruginosa (gram-negative environmental bacilli associated with water systems). In addition, it must be certified as virucidal and fungicidal.
  • Tuberculocidal Disinfectants: These specialized disinfectants are clinically certified to kill Mycobacterium tuberculosis. The tuberculosis bacillus possesses a dense, waxy, lipid-rich outer cell wall that makes it exceptionally resilient against common germicides. A disinfectant proven effective against tuberculosis is classified as a higher-level disinfectant. Under many state board regulations, tuberculocidal disinfectants are required when cleaning up blood exposure incidents or contaminated implements.

Contact and Immersion Times

A disinfectant cannot kill microorganisms instantaneously; it requires adequate chemical exposure duration, termed the contact time:

  • Complete Immersion: For nonporous multi-use tools (shears, clippers, combs, cuticle nippers), state licensing guidelines require complete immersion in an EPA-registered liquid disinfectant for a minimum of 10 minutes (unless an EPA-approved manufacturer label specifies a shorter validated contact time).
  • Surface Sprays & Wipes: When disinfecting countertops, shampoo bowls, and styling chairs, the chemical spray or wipe must leave the surface visibly wet for the full contact time stated on the product label (typically ranging from 2 to 10 minutes). Wiping a surface dry immediately after spraying negates the disinfection process.

Porous vs. Nonporous & Single-Use vs. Multi-Use Implements

Salon tools are strictly divided based on their physical porosity and reusable status:

+-----------------------------------------------------------------------------------------+
|                         IMPLEMENT CLASSIFICATION STANDARDS                              |
+-------------------+--------------------+------------------------+-----------------------+
| Classification    | Physical Material  | Regulatory Mandate     | Examples              |
+-------------------+--------------------+------------------------+-----------------------+
| Nonporous         | Metal, hard plastic| Clean, disinfect, and  | Stainless shears,     |
| (Multi-Use)       | glass, ceramic     | reuse between every    | plastic combs, steel  |
|                   |                    | client                 | nippers, acrylic rods |
+-------------------+--------------------+------------------------+-----------------------+
| Porous / Absorbent| Wood, cardboard,   | Discard in covered     | Wooden orange sticks, |
| (Single-Use)      | paper, foam, cotton| trash immediately      | emery boards, buffer  |
|                   |                    | after a single service | blocks, cotton balls  |
+-------------------+--------------------+------------------------+-----------------------+
  • Nonporous Implements (Multi-Use): Made of hard, dense materials that have no pores or openings and cannot absorb liquids. Examples include stainless steel shears, carbon-fiber combs, metal cuticle pushers, and hard plastic rollers. These tools can be washed with detergent, dried, fully submerged in chemical disinfectants, and reused indefinitely.
  • Porous Implements (Single-Use): Made of permeable, absorbent materials with microscopic voids that can absorb and trap moisture, bacteria, fungal spores, and sebum. Examples include wooden orange sticks, cardboard emery boards, foam toe separators, cotton rounds, neck strips, and pumice stones. Because porous materials cannot be completely cleared of absorbed fluids and cannot withstand immersion in chemical disinfectants without degrading, they are classified as single-use (disposable) and must be discarded into a covered trash receptacle immediately after one service.
  • Blood-Contaminated Single-Use Items: If a disposable porous item contacts blood or body fluids, it must be placed into a sealed plastic bag (double-bagged) before disposal in standard waste, or deposited into a designated biohazard container in compliance with OSHA standards.

Autoclave Operation & Biological Spore Testing

For salons and medical spas that perform advanced aesthetic services requiring tool sterilization, the steam autoclave is the gold standard.

Autoclave Operating Parameters

An autoclave utilizes saturated steam under pressure to denature microbial proteins and destroy nucleic acids. Standard cycle parameters require maintaining a chamber temperature of 121°C (250°F) at 15 pounds per square inch (psi) of pressure for 15 to 30 minutes, or 132°C (270°F) at 30 psi for 3 to 10 minutes for rapid-cycle sterilization. Implements must be pre-cleaned, dried, and sealed in specialized sterilization pouches with heat-sensitive process indicators before entering the chamber.

Sterilization Monitoring & Spore Testing

Sterilization efficacy must be verified through three levels of monitoring:

  1. Mechanical Monitoring: Observing and documenting chamber temperature, pressure gauges, and cycle run times.
  2. Chemical Indicators: Heat-sensitive chemical indicator tape or pouch markings that change color upon exposure to heat and steam.

    Critical Distinction: Chemical indicators only prove that the package was exposed to heat; they do not confirm that microbial death occurred.

  3. Biological Monitoring (Spore Testing): The only definitive, scientifically validated method to prove that an autoclave is achieving sterilization.
    • Sealed ampoules or paper strips containing live, heat-resistant bacterial endospores of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) are processed through an ordinary autoclave cycle.
    • The processed spores are incubated alongside an unprocessed control vial.
    • If the processed spores fail to grow, sterilization is verified.
    • State health boards and the CDC recommend regular (weekly or monthly) biological spore testing conducted through an accredited mail-in testing laboratory, with all physical documentation maintained in the salon logbook for regulatory inspection.
Test Your Knowledge

Under CDC Standard Precautions, which human bodily fluid is the ONLY secretion excluded from the assumption of infectious risk, provided it contains no visible blood?

A
B
C
D
Test Your Knowledge

Why must a cosmetologist thoroughly wash with soap, rinse, and dry shears or implements before submerging them into an EPA-registered disinfectant solution?

A
B
C
D
Test Your Knowledge

Which monitoring method provides the only definitive, legally recognized biological proof that a salon autoclave has successfully achieved sterilization?

A
B
C
D