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100+ Free Biology 30 Diploma Practice Questions

Pass your Alberta Biology 30 Diploma Examination exam on the first try — instant access, no signup required.

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Question 1
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In a dihybrid cross between two individuals heterozygous for both traits (RrYy x RrYy), what is the expected phenotypic ratio of the offspring, assuming independent assortment?

A
B
C
D
to track
2026 Statistics

Key Facts: Biology 30 Diploma Exam

The Biology 30 diploma is a 60-question (48 multiple-choice + 12 numerical-response) Grade 12 Alberta exam written in 3 hours, worth 30% of the final mark, with an acceptable standard of 50% and excellence at 80%.

Sample Biology 30 Diploma Practice Questions

Try these sample questions to test your Biology 30 Diploma exam readiness. Each question includes a detailed explanation. Start the interactive quiz above for the full 100+ question experience with AI tutoring.

1In a resting neuron, the inside of the membrane is negatively charged relative to the outside. What is the approximate value of this resting membrane potential?
A.+35 mV
B.-70 mV
C.0 mV
D.+70 mV
Explanation: A resting neuron maintains a resting membrane potential of approximately -70 mV, meaning the inside of the membrane is more negative than the outside. This is maintained by the sodium-potassium pump and the membrane's selective permeability to potassium ions.
2During the depolarization phase of an action potential, which ion movement is primarily responsible for the change in membrane potential?
A.Potassium (K+) moving out of the neuron
B.Sodium (Na+) moving into the neuron
C.Chloride (Cl-) moving into the neuron
D.Calcium (Ca2+) moving out of the neuron
Explanation: Depolarization occurs when voltage-gated sodium channels open and Na+ rushes into the neuron, making the inside more positive. This rapid influx drives the membrane potential toward about +35 mV.
3The sodium-potassium pump in a neuron uses ATP to actively transport ions against their concentration gradients. For each cycle, how many ions are moved?
A.3 Na+ out and 2 K+ in
B.2 Na+ out and 3 K+ in
C.3 K+ out and 2 Na+ in
D.2 Na+ in and 3 K+ out
Explanation: The sodium-potassium pump moves 3 Na+ ions out of the cell and 2 K+ ions into the cell per ATP hydrolyzed. This unequal exchange helps maintain the negative resting potential and the ion gradients used during action potentials.
4A neurotransmitter released into the synaptic cleft binds to receptors on the postsynaptic membrane. Which neurotransmitter is broken down by acetylcholinesterase at the neuromuscular junction?
A.Dopamine
B.Serotonin
C.Acetylcholine
D.Norepinephrine
Explanation: Acetylcholine is the neurotransmitter at the neuromuscular junction, and it is broken down by the enzyme acetylcholinesterase. This enzymatic breakdown terminates the signal so the muscle can relax and respond to new impulses.
5Which part of the human brain is responsible for coordinating voluntary movement, balance, and muscle coordination?
A.Cerebrum
B.Medulla oblongata
C.Hypothalamus
D.Cerebellum
Explanation: The cerebellum coordinates voluntary muscle movements, balance, and posture. It refines motor commands originating in the cerebrum so that movements are smooth and coordinated.
6In a reflex arc such as the knee-jerk reflex, which sequence correctly traces the pathway of a nerve impulse?
A.Motor neuron, interneuron, sensory neuron, effector
B.Sensory neuron, interneuron, motor neuron, effector
C.Effector, motor neuron, sensory neuron, receptor
D.Receptor, motor neuron, interneuron, sensory neuron
Explanation: A reflex arc begins at a receptor, travels along a sensory (afferent) neuron to the spinal cord, may pass through an interneuron, then exits via a motor (efferent) neuron to an effector such as a muscle. This pathway allows rapid responses without conscious brain involvement.
7Light entering the eye is focused on the retina, which contains photoreceptors. Which photoreceptors are responsible for colour vision in bright light?
A.Rods
B.Ganglion cells
C.Bipolar cells
D.Cones
Explanation: Cones are the photoreceptors responsible for colour vision and function best in bright light. There are three types of cones sensitive to different wavelengths, allowing the perception of colour.
8When you focus on a nearby object, the lens of the eye becomes more rounded. What is this process of adjusting lens shape called?
A.Refraction
B.Adaptation
C.Dilation
D.Accommodation
Explanation: Accommodation is the process by which the ciliary muscles change the shape of the lens to focus on objects at different distances. For near objects, the ciliary muscles contract and the lens becomes more rounded.
9The structure in the inner ear that is responsible for converting sound vibrations into nerve impulses is the:
A.Cochlea
B.Eustachian tube
C.Tympanic membrane
D.Semicircular canals
Explanation: The cochlea is the fluid-filled, spiral structure of the inner ear that contains hair cells. When sound vibrations move through the cochlear fluid, the hair cells convert these vibrations into nerve impulses sent to the brain.
10The semicircular canals of the inner ear are primarily responsible for detecting which type of information?
A.Sound frequency
B.Light intensity
C.Rotational movement and dynamic equilibrium
D.Air pressure changes
Explanation: The three semicircular canals are oriented in different planes and detect rotational movements of the head, contributing to dynamic equilibrium. Movement of fluid within them stimulates hair cells that signal the brain about head position changes.

About the Biology 30 Diploma Exam

The Alberta Biology 30 Diploma Examination is a Grade 12 provincial exam set by Alberta Education that assesses the Biology 30 program of studies across four units: Nervous and Endocrine Systems, Reproduction and Development, Cell Division/Genetics/Molecular Biology, and Population and Community Dynamics. The real exam consists of 60 machine-scored questions (48 multiple-choice and 12 numerical-response of equal value) written in 3 hours, with up to 6 hours permitted. It is closed-book, offered in English only, and an approved calculator may be used. The diploma exam is weighted at 30% of a student's final course mark, with the school-awarded mark making up the other 70%. Results count toward the Alberta High School Diploma and post-secondary admission, and questions range across remembering/understanding, applying, and higher mental activity cognitive levels.

Questions

100 scored questions

Time Limit

3 hours designed time (up to 6 hours allowed)

Passing Score

Acceptable standard 50%; standard of excellence 80%. Worth 30% of the final course mark.

Exam Fee

Free for first-time funded Alberta students; CAD $26.25 rewrite fee (incl. GST); CAD $50.00 per exam for non-funded / international students. (Alberta Education, Provincial Assessment Sector (Government of Alberta))

Biology 30 Diploma Exam Content Outline

25%

Unit A: Nervous and Endocrine Systems

Neurons, resting and action potentials, synapses and neurotransmitters, CNS and PNS, the eye and ear, hormones, and homeostasis including blood glucose regulation.

20%

Unit B: Reproduction and Development

Male and female reproductive systems, hormonal regulation and the menstrual cycle, gametogenesis, fertilization, embryonic development, and reproductive technologies.

40%

Unit C: Cell Division, Genetics and Molecular Biology

Mitosis and meiosis, Mendelian and dihybrid crosses, sex linkage, pedigrees, DNA structure and replication, protein synthesis, mutations, and genetic technology.

15%

Unit D: Population and Community Dynamics

Population growth, carrying capacity, Hardy-Weinberg equilibrium, evolution and natural selection, community interactions, and ecological succession.

How to Pass the Biology 30 Diploma Exam

What You Need to Know

  • Passing score: Acceptable standard 50%; standard of excellence 80%. Worth 30% of the final course mark.
  • Exam length: 100 questions
  • Time limit: 3 hours designed time (up to 6 hours allowed)
  • Exam fee: Free for first-time funded Alberta students; CAD $26.25 rewrite fee (incl. GST); CAD $50.00 per exam for non-funded / international students.

Keys to Passing

  • Complete 500+ practice questions
  • Score 80%+ consistently before scheduling
  • Focus on highest-weighted sections
  • Use our AI tutor for tough concepts

Biology 30 Diploma Study Tips from Top Performers

1Prioritize Unit C (Cell Division, Genetics and Molecular Biology), which carries the heaviest weight (~40%); practise monohybrid, dihybrid, sex-linked, and pedigree problems until they are automatic.
2Master Hardy-Weinberg calculations using p + q = 1 and p^2 + 2pq + q^2 = 1, and memorize the five conditions for genetic equilibrium.
3Practise numerical-response questions specifically, since 12 of the real exam's 60 questions require you to calculate and grid in numeric answers.
4Use the Biology 30 Data Booklet and released diploma exams from Alberta Education to learn the exam's context-dependent question style.
5Diagram and label key structures (neuron, eye, ear, reproductive systems) and trace pathways such as the reflex arc and hormonal feedback loops.
6Build a hormone chart linking each hormone to its gland, target, and effect, and practise distinguishing negative from positive feedback.

Frequently Asked Questions

How many questions are on the Biology 30 diploma exam?

The real diploma exam has 60 machine-scored questions of equal value: 48 multiple-choice and 12 numerical-response. This free practice bank provides 100 questions to help you prepare across all four units.

How much is the Biology 30 diploma exam worth?

The diploma exam is weighted at 30% of your final course mark, and your school-awarded mark makes up the other 70%. You need a combined final mark of at least 50% to pass the course.

What is the passing score for the Biology 30 diploma exam?

Alberta Education sets the acceptable standard at 50% and the standard of excellence at 80%. There is no separate 'pass' on the diploma alone; your final mark blends the 30% exam with your 70% school mark.

How long is the Biology 30 diploma exam?

The exam is designed to be completed in 3 hours, but students may take up to 6 hours if they need the extra time. It is a closed-book exam offered in English only, and an approved calculator is permitted.

What units does the Biology 30 diploma exam cover?

It covers four units: Unit A Nervous and Endocrine Systems (~25%), Unit B Reproduction and Development (~20%), Unit C Cell Division, Genetics and Molecular Biology (~40%), and Unit D Population and Community Dynamics (~15%).

Is there a fee to write the Biology 30 diploma exam?

First-time funded Alberta students write for free. Rewriters pay a CAD $26.25 fee (incl. GST), and non-funded visiting or international students pay CAD $50.00 per exam (incl. GST), registered through myPass.