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100+ Free AEPA Biology (NT305) Practice Questions

Pass your Arizona Educator Proficiency Assessments Biology (NT305) Subject Knowledge Test exam on the first try — instant access, no signup required.

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A change in the nucleotide sequence of DNA is called a:

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Key Facts: AEPA Biology (NT305) Exam

NT305

Current AEPA Biology Code

AEPA tests list

150

Multiple-Choice Questions

AEPA Biology test page

3h

Testing Time

AEPA Biology test page

220

Passing Score

AEPA Biology test page

$119

Test Fee

AEPA Biology test page

20/13/27/20/20

Official Domain Weights

AEPA/NES Biology profile

AEPA currently lists Biology as test code NT305. The Biology test is a Pearson-delivered National Evaluation Series assessment with 150 multiple-choice questions, 3 hours of testing time within a 3-hour-15-minute appointment, a passing score of 220, and a posted fee of $119. The official profile weights the domains at 20% Nature of Science, 13% Biochemistry and Cell Biology, 27% Genetics and Evolution, 20% Biological Unity and Diversity, and 20% Ecology and Environment.

Sample AEPA Biology (NT305) Practice Questions

Try these sample questions to test your AEPA Biology (NT305) exam readiness. Each question includes a detailed explanation. Start the interactive quiz above for the full 100+ question experience with AI tutoring.

1A biology teacher asks students whether soil pH affects radish seed germination. In a controlled experiment, which variable should students deliberately change?
A.The number of seeds placed in each dish
B.The temperature of the growth chamber
C.The pH of the soil or growth medium
D.The percentage of seeds that germinate
Explanation: The independent variable is the factor intentionally changed to test its effect. Because the question asks whether pH affects germination, pH is the variable to manipulate. Seed number and temperature should be controlled, and germination percentage is the response being measured.
2In an experiment testing a new fertilizer, why should one group of plants receive no fertilizer?
A.To provide a baseline for comparison with fertilized plants
B.To make sure every plant receives the same treatment
C.To prove the fertilizer works before data are collected
D.To eliminate the need for repeated trials
Explanation: A control group provides a reference point for interpreting the effect of the independent variable. If unfertilized and fertilized plants differ, the comparison helps determine whether the fertilizer was associated with the change. Replication and careful controls are still needed.
3Which statement is the best example of a scientific claim?
A.This ecosystem is the most beautiful one in the region.
B.Students should enjoy studying photosynthesis.
C.Increasing nitrate concentration increased algal growth in three replicated pond tanks.
D.Wetlands are more interesting than deserts.
Explanation: Scientific claims are testable and supported by evidence. The nitrate statement identifies a measurable relationship and refers to replicated observations. The other statements express preferences or values rather than evidence-based explanations.
4A student spills a live bacterial culture on a lab bench. Which first response is most appropriate?
A.Wipe it with a dry paper towel and continue the lab.
B.Notify the teacher and follow the classroom biological spill procedure.
C.Smell the culture to identify the organism.
D.Pour the culture into the sink with running water.
Explanation: Biological spills require prompt reporting and approved decontamination procedures. The teacher can ensure that the correct disinfectant, contact time, and disposal method are used. Students should not handle unknown biological spills casually.
5A bacterium is about 0.000002 meters long. Which notation expresses this length in scientific notation?
A.2 x 10^-6 m
B.2 x 10^-5 m
C.2 x 10^6 m
D.0.2 x 10^-6 m
Explanation: Moving the decimal in 0.000002 six places to the right gives 2, so the exponent is -6. Scientific notation uses a coefficient from 1 up to, but not including, 10. Therefore the correct expression is 2 x 10^-6 m.
6Why are models such as food webs useful but limited in biology?
A.They can represent important relationships while simplifying many details of real systems.
B.They are exact copies of ecosystems and need no revision.
C.They replace the need for observations in natural environments.
D.They show only incorrect ideas that scientists must reject.
Explanation: Scientific models help represent complex systems, make predictions, and organize evidence. They are limited because they simplify reality and may omit variation, scale, or changing conditions. Models are revised when new evidence shows they are incomplete.
7A field ecologist estimates insect diversity in a meadow by sampling only the plants nearest the parking lot. Which concern most directly weakens the investigation?
A.The sample may be biased because it is not representative of the whole meadow.
B.The investigation uses living organisms, so it cannot produce scientific evidence.
C.The number of insect species is a controlled variable.
D.All field studies must include a microscope.
Explanation: Sampling only near a parking lot can overrepresent disturbed edge conditions and underrepresent the rest of the meadow. A representative or randomized sampling plan would support stronger inferences about the whole site. Sampling design is central to ecological data quality.
8Students test catalase activity at several temperatures and find the highest reaction rate at 37 degrees C, with lower rates at 10 degrees C and 70 degrees C. Which conclusion is best supported?
A.Catalase functions best at an intermediate temperature under the tested conditions.
B.Catalase activity always increases as temperature increases.
C.Temperature has no effect on catalase activity.
D.The enzyme was destroyed at every temperature tested.
Explanation: The data show a peak at 37 degrees C rather than a steady increase or no change. Enzymes commonly have optimal temperature ranges because low temperature reduces molecular motion and high temperature can disrupt protein structure. The conclusion should stay within the tested conditions.
9A news headline says, "Eating one berry prevents all viral infections," based on a study of 12 volunteers who self-reported fewer colds. Which critique is most scientifically appropriate?
A.The claim is stronger than the evidence because the sample is small and self-reported.
B.The claim is certainly true because the study involved humans.
C.The study proves that berries kill every virus in the body.
D.The result should be rejected only because it involves nutrition.
Explanation: Scientific media claims should be evaluated for sample size, controls, measurement quality, and whether conclusions exceed the data. A small self-reported study cannot justify a universal prevention claim. The critique targets the evidential support, not the topic itself.
10A student tests whether light color affects plant growth. Which condition should be held constant across treatment groups?
A.The color of light reaching each group
B.The height increase measured after two weeks
C.The species and starting size of the plants
D.The conclusion drawn from the data
Explanation: Controlled variables are factors kept the same so the independent variable can be interpreted. Plant species and starting size can affect growth, so they should be held constant. Light color is the independent variable, and height increase is the dependent variable.

About the AEPA Biology (NT305) Exam

AEPA Biology (NT305) is the current Arizona subject knowledge assessment for Biology teacher certification. The Pearson/NES profile organizes the exam into five weighted domains: Nature of Science, Biochemistry and Cell Biology, Genetics and Evolution, Biological Unity and Diversity, and Ecology and Environment.

Assessment

150 multiple-choice questions

Time Limit

3h testing time (3h 15m appointment)

Passing Score

220 scaled score

Exam Fee

$119 (Arizona Educator Proficiency Assessments / Pearson)

AEPA Biology (NT305) Exam Content Outline

20%

Nature of Science

Scientific inquiry, experimental design, data collection and interpretation, evidence-based claims, scientific notation, safety, history and nature of science, and biology's connections with technology, mathematics, engineering, and society.

13%

Biochemistry and Cell Biology

Atoms, molecules, water, carbon, chemical bonds, thermodynamics, macromolecules, enzymes, membranes, organelles, homeostasis, photosynthesis, cellular respiration, cell specialization, binary fission, mitosis, and the cell cycle.

27%

Genetics and Evolution

Nucleic acids, gene expression, protein synthesis, mutations, genetic engineering, meiosis, inheritance patterns, chromosomal inheritance, extranuclear inheritance, Hardy-Weinberg reasoning, natural and artificial selection, speciation, evidence for evolution, classification, phylogenetic trees, and the history of life.

20%

Biological Unity and Diversity

Viruses, prokaryotes, protists, fungi, plants, animals, reproduction and life cycles, organism energy and matter use, plant and animal homeostasis, and the anatomy, physiology, disorders, and disease prevention of major human organ systems.

20%

Ecology and Environment

Biotic and abiotic population limits, density-dependent and density-independent factors, behavior and social systems, demographics and survivorship, community interactions, niches, succession, ecosystems, biomes, trophic roles, biogeochemical cycles, and effects of human activities on aquatic, terrestrial, and atmospheric systems.

How to Pass the AEPA Biology (NT305) Exam

What You Need to Know

  • Passing score: 220 scaled score
  • Assessment: 150 multiple-choice questions
  • Time limit: 3h testing time (3h 15m appointment)
  • Exam fee: $119

Keys to Passing

  • Complete 500+ practice questions
  • Score 80%+ consistently before scheduling
  • Focus on highest-weighted sections
  • Use our AI tutor for tough concepts

AEPA Biology (NT305) Study Tips from Top Performers

1Study by official weighting: give the most time to Genetics and Evolution, then keep Nature of Science, Unity and Diversity, and Ecology on equal footing.
2Practice experimental design and data interpretation because Nature of Science items often test evidence, variables, controls, and safe lab procedures rather than memorized facts alone.
3For cellular topics, connect structure to function: membranes, organelles, enzymes, photosynthesis, respiration, mitosis, and homeostasis.
4For genetics, practice meiosis, pedigrees, probability, gene expression, mutations, and Hardy-Weinberg reasoning until the relationships are automatic.
5For diversity and ecology, focus on adaptations, life cycles, energy flow, matter cycling, population regulation, and human impacts across biological scales.

Frequently Asked Questions

What is the current AEPA Biology test code?

The current AEPA tests list shows Biology as NT305. This matters because older or unofficial lists may use stale code data, but the current official AEPA list and Biology pages identify the test as Biology (305) / NT305.

How many questions are on AEPA Biology NT305?

The official test page lists 150 multiple-choice questions. The profile page also describes the test format as multiple-choice questions and lists approximately 150 questions.

How long is the AEPA Biology exam?

Candidates receive 3 hours of testing time. The total appointment is 3 hours and 15 minutes because the tutorial and nondisclosure agreement take up to 15 minutes.

What score do I need to pass AEPA Biology?

The official AEPA Biology test page lists a passing score of 220. Scores are reported on AEPA's scaled-score system.

How much does AEPA Biology cost?

The current official AEPA Biology NT305 test page lists a test fee of $119. Candidates should still verify the final checkout amount before registering in case fees change.

Which AEPA Biology domain should I study most?

Genetics and Evolution is the largest official domain at 27% of the test. Nature of Science, Biological Unity and Diversity, and Ecology and Environment are each 20%, while Biochemistry and Cell Biology is 13%.