3.2 Physiologic Signals & Calibrations
Key Takeaways
- A diagnostic PSG records EEG, electrooculogram (EOG), chin and limb electromyogram (EMG), electrocardiogram (ECG), airflow (oronasal thermistor plus nasal pressure transducer), respiratory effort (respiratory inductance plethysmography, RIP), oxygen saturation (SpO2), snore, and body position.
- Apneas are scored using the oronasal thermistor; hypopneas are scored using the nasal pressure transducer because it is more sensitive to subtle airflow reductions.
- Physiologic (bio-) calibrations have the patient blink, look in cardinal directions, grit teeth, breathe, and move limbs to confirm each channel works before lights out.
- AASM filter settings: EEG/EOG low-frequency filter (LFF) 0.3 Hz and high-frequency filter (HFF) 35 Hz; EMG LFF 10 Hz with HFF 100 Hz; ECG LFF 0.3 Hz with HFF 70 Hz.
- An all-channel electrical (square-wave) calibration verifies that every amplifier responds identically before sensors are connected.
The Diagnostic PSG Signal Array
A diagnostic polysomnogram (PSG) is a multi-parameter recording. The RPSGT exam expects you to know what each channel measures, which sensor scores which event, and how to confirm the signal is valid. The full AASM recommended array is summarized below.
| Signal | Sensor | Primary Use |
|---|---|---|
| EEG | Scalp electrodes (10-20 sites) | Sleep staging, arousals |
| EOG | Electrodes near outer canthi (E1, E2) | Eye movements: REM, slow eye movements |
| Chin EMG | Submental (chin) electrodes | Muscle tone; REM atonia, bruxism |
| Limb EMG | Anterior tibialis electrodes | Periodic limb movements (PLMs) |
| ECG | Modified Lead II (torso) | Heart rate and arrhythmia |
| Airflow | Oronasal thermistor + nasal pressure transducer | Apneas (thermistor) and hypopneas (pressure) |
| Effort | Respiratory inductance plethysmography (RIP) thorax + abdomen belts | Obstructive vs. central events |
| SpO2 | Pulse oximeter (finger probe) | Oxygen desaturation |
| Snore | Snore microphone or piezo sensor | Snoring documentation |
| Body position | Position sensor | Supine vs. lateral event distribution |
The EOG channels are intentionally placed off-axis: E1 is 1 cm below the left outer canthus and E2 is 1 cm above the right outer canthus, both referenced to a mastoid. This offset makes conjugate eye movements appear out of phase between the two channels — the signature used to confirm rapid eye movements in REM and to distinguish true eye movement from EEG artifact.
Airflow: Thermistor vs. Nasal Pressure
This distinction is heavily tested:
- Oronasal thermistor detects temperature change between inhaled and exhaled air. It captures oral and nasal airflow and is the AASM sensor for scoring apneas (a ≥90% drop in the thermistor signal for ≥10 seconds).
- Nasal pressure transducer measures pressure swings and is far more sensitive to subtle airflow limitation. It is the AASM sensor for scoring hypopneas (a ≥30% drop for ≥10 seconds with an associated ≥3% desaturation or arousal) and for detecting flow limitation and the flattened plateau that signals upper-airway resistance.
If the thermistor fails, the nasal pressure signal (or RIP sum) is an acceptable backup for apnea scoring, and vice versa — a substitution the technologist must log.
Calibrations
Calibrations prove the equipment and sensors work before you commit a night of recording. There are two distinct kinds, and the exam tests the difference.
1. Electrical (Square-Wave) Calibration
An equal calibration voltage (for example, a 50 µV square wave) is applied to every channel before sensors are connected. Each amplifier should produce an identical, well-formed waveform with matching amplitude and the same rise and decay (time constant). Differences reveal a faulty amplifier, a wrong gain, or a misset filter. This is a test of the recording system, not the patient, and it confirms gains and filters are uniform across channels so that a 50 µV deflection means the same thing on every trace.
2. Physiologic (Bio-) Calibration
With all sensors attached and the patient awake, the technologist directs specific maneuvers and watches the screen to confirm each channel responds correctly:
| Command | Channel verified | Expected response |
|---|---|---|
| "Eyes open, look straight ahead" | Occipital EEG | Low-voltage, mixed-frequency baseline |
| "Eyes closed" | Occipital EEG | Posterior dominant alpha rhythm increases |
| "Look up / down / left / right" | EOG (E1, E2) | Out-of-phase deflections |
| "Blink five times" | Frontal EEG / EOG | Sharp synchronous deflections |
| "Grit your teeth" | Chin (submental) EMG | Clear amplitude (tone) increase |
| "Breathe in, hold, breathe out" | Airflow + effort | In-phase respiratory deflections |
| "Flex right then left foot" | Limb EMG | Burst on the corresponding leg channel |
The bio-calibration confirms each sensor is attached, correctly referenced, and reading from the intended source. Document it in the technologist log. Any channel that does not respond — a flat EOG on lateral gaze, a silent chin EMG on teeth-gritting — must be corrected before lights out, because these are the very channels REM and arousal scoring depend on.
Filters & Sensitivities
Filters shape which frequencies are displayed; sensitivity (gain) scales amplitude. The AASM publishes recommended settings so studies are comparable between labs.
| Channel | Low-Frequency Filter (LFF) | High-Frequency Filter (HFF) |
|---|---|---|
| EEG | 0.3 Hz | 35 Hz |
| EOG | 0.3 Hz | 35 Hz |
| EMG (chin/limb) | 10 Hz | 100 Hz |
| ECG | 0.3 Hz | 70 Hz |
| Airflow/effort | 0.1 Hz | 15 Hz |
| Snore | 10 Hz | 100 Hz |
Note the logic: the EMG LFF is set high (10 Hz) to roll off slow movement and sweat drift while preserving fast muscle bursts, whereas the EEG LFF is set low (0.3 Hz) so slow-wave (delta) activity survives. The airflow/effort HFF is low (15 Hz) because respiration is a slow signal and higher frequencies are only noise.
A 60 Hz notch filter (mains/line filter) should be the last resort for electrical noise — it can mask a real impedance or grounding problem and distorts nearby frequencies. Always correct the cause (re-prep electrode, secure the patient ground, remove an interfering device) before applying a notch filter.
Sensitivity
Typical sensitivities: EEG roughly 7 microvolts per millimeter (µV/mm), EOG ~7 µV/mm, chin EMG ~5 µV/mm or finer, ECG much larger (~1 mV scale). A key rule the exam tests: sensitivity changes only the displayed amplitude, not the underlying digitized data. Lowering EEG sensitivity to make 60 Hz artifact "look smaller" hides the problem without fixing it and can flatten a genuine slow-wave or K-complex you needed to score.
Common Trap
Applying the EMG filter (LFF 10 Hz) to an EEG channel attenuates delta and theta activity — the study will under-stage N3 and may misread true slow-wave sleep as lighter stages. Match the filter table to the channel, every time.
Which sensor does the AASM specify for scoring obstructive apneas, and which for hypopneas?
During physiologic (bio-) calibration, you ask the patient to grit their teeth. Which channel should show the expected response?
The recommended AASM low-frequency filter (LFF) and high-frequency filter (HFF) for EEG channels are:
Persistent 60 Hz electrical artifact appears across several EEG channels at the start of a study. What is the most appropriate first action?