9.1 Department Delivery

Key Takeaways

  • Route specimens by order and tube type—chemistry (serum/plasma), hematology (EDTA), coagulation (citrate), microbiology (cultures and non-blood), and blood bank (strict labeling)—not by convenience
  • Light-blue citrate tubes go to coagulation; lavender EDTA to hematology; SST/gold/red/green typically to chemistry; blood culture bottles and non-blood cultures to microbiology
  • Blood bank specimens require two unique identifiers, collector signature, date/time, and often a second person verification—never relabel or combine tubes for transfusion workups
  • STAT, timed, and irreplaceable specimens (CSF, blood cultures, ABGs) bypass routine batching and follow hand-delivery or priority routing per facility policy
  • Central receiving may sort multi-department draws, but packaging must still prevent leaks, protect temperature, and keep paperwork dry and readable
Last updated: July 2026

Domain II-D—select proper department for specimen delivery—tests whether you can move a labeled specimen from the bedside to the bench that actually performs the ordered test. Hospitals may use central accessioning, pneumatic tube stations, or direct walk-in delivery, but the routing logic is the same: tube additive, specimen type, and order determine destination. Sending a coagulation tube to hematology because "it is purple-ish" or routing a type-and-screen to chemistry because "it is blood" creates delays, redraws, and patient-safety risk.

Why Department Routing Is a Safety Competency

Laboratory sections use different instruments, reagents, and quality-control rules. A CBC analyzer cannot run a PT/INR on citrate plasma without a different workflow. Microbiology incubates living organisms; chemistry measures analytes in serum or plasma. Blood bank work can affect transfusion decisions—misidentified tubes there are never "close enough." Your delivery decision is the last preanalytical gate before the specimen enters a method that assumes you sent the right material to the right place.

Think in three steps every time:

  1. Read the order (electronic or paper requisition).
  2. Match the container you actually collected.
  3. Deliver to the section (or central receiving) that processes that combination.

If step 2 and step 1 disagree—wrong tube drawn, missing culture bottle—stop and escalate before delivery.

Chemistry Department

Chemistry performs quantitative and qualitative assays on serum, plasma, or whole blood depending on the test. Typical collection tubes include:

Tube / specimenCommon chemistry usesDelivery notes
Gold/SST (serum separator)CMP, BMP, lipids, liver panel, many enzymesAllow clot if required; centrifuge per policy before or after delivery to central lab
Red-top (no additive)Serum tests when SST unavailableClot, centrifuge, separate serum if policy requires
Green-top (lithium or sodium heparin)Plasma chemistry, some STAT panels, blood gases in some systemsDo not confuse with light-blue citrate—green is heparin, not coagulation PT
Gray-top (fluoride/oxalate or fluoride only)Glucose, lactate (with ice)Lactate and some glucose protocols need immediate ice and STAT routing
Urine (random, timed, 24-hr)Urinalysis, urine chemistry, microalbuminSeparate from blood tubes; follow preservative and refrigeration rules

Chemistry is often the highest-volume destination. Many facilities route all "routine blood tubes" to central specimen processing, which centrifuges and aliquots. Even then, you must not dump incompatible specimens in one leaking bag—separate iced ammonia from room-temperature CBC tubes, and never place blood bank tubes in a routine chemistry carrier without blood-bank policy approval.

High-yield chemistry delivery traps:

  • Sending a light-blue citrate tube to chemistry for PT/INR (belongs in coagulation).
  • Delivering unspun SST for a test that requires serum when the lab expects spun tubes at receipt.
  • Mixing iced specimens with ambient tubes so ice melts and warms critical analytes.

Hematology Department

Hematology focuses on cellular components of blood—primarily EDTA (lavender-top) whole blood.

Test categoryTypical tubeDelivery notes
CBC with differentialLavender EDTAMix gently by inversion 8–10 times at collection; deliver within stability window
ESR, reticulocyte countLavender EDTAAvoid extreme shaking that can alter platelets or red-cell indices
Hemoglobin A1cLavender EDTA (facility-dependent; some use lavender or dedicated tube)Follow local directory—do not assume gold-top
Peripheral blood smear reviewLavender EDTASome labs want slides made at bedside; deliver promptly

Hematology specimens are sensitive to clotting (if EDTA inadequate or tube underfilled), agitation, and delay. A lavender tube left in a hot car or vigorously shaken in a packed carrier can produce falsely abnormal platelet counts. Keep tubes upright when possible and deliver within the laboratory's stated stability limit—often a few hours at room temperature for CBC, shorter for some specialty hematology assays.

Do not send lavender tubes to coagulation for "any blood test" or to chemistry unless the order explicitly lists a chemistry method on EDTA whole blood.

Coagulation Department

Coagulation uses sodium citrate (light-blue-top) tubes filled to the exact fill line so the 9:1 blood-to-anticoagulant ratio is correct.

TestTubeCritical delivery points
PT/INR, aPTTLight-blue citrateUnderfilled = rejected; deliver promptly—some assays unstable if delayed
Fibrinogen, D-dimer, factor assaysLight-blue citrateMay require centrifugation at specific g-force; follow STAT rules
Platelet function, TEG/ROTEM (facility-specific)Specialized tubesOften excluded from pneumatic tube transport—hand carry

Coagulation is unforgiving about volume and time. A tube drawn to half the line will be rejected before it reaches the analyzer. If you must batch deliveries, coagulation STATs and timed draws still outrank routine chemistry drops.

Exam contrast: Green heparin ≠ light-blue citrate. Heparin inhibits clotting by a different mechanism and is used for plasma chemistry, not standard PT/INR on citrate instruments.

Microbiology Department

Microbiology receives specimens that must stay viable for culture or that require direct microscopic examination—blood culture bottles, urine for culture, sputum, stool, wound swabs, throat swabs, and other site-specific cultures.

Specimen typeTypical containerDelivery priority
Blood culturesAerobic/anaerobic bottlesSTAT—incubate ASAP; do not refrigerate routine bottles unless manufacturer says so
Urine cultureSterile cup or preservative per orderDeliver within 1–2 hours when possible; refrigerate if delay
Sputum, wound, throat swabsSterile cup or transport mediumKeep transport media moist; avoid temperature extremes
Stool culture / O&PClean container or preservative vialO&P needs preservative promptly; some enteric cultures must not be refrigerated

Microbiology is not the default for "any tube with red liquid." A gold-top serum tube for hepatitis serology may go to serology/immunology or chemistry depending on the lab layout—read the order. True culture specimens almost always route to microbiology or a dedicated bench listed on the requisition.

Never delay blood cultures while you finish paperwork for routine chemistries. Two sets from different sites mean nothing if bottles sit at room temperature in a drawer for hours against policy.

Blood Bank / Transfusion Services

Blood bank specimens support typing, antibody screening, crossmatching, and transfusion reaction workups. Requirements are stricter than routine chemistry:

  • Two unique patient identifiers on the tube and requisition, matching exactly.
  • Collector signature and date/time of collection—often bedside labeling mandatory.
  • Second-person verification at many facilities for transfusion-related collections.
  • Dedicated pink-top or facility-specified EDTA tubes for some type-and-screen protocols.
  • Hand delivery or designated blood-bank carriers—many policies prohibit mixing these tubes in generic pneumatic sends.

Blood bank will reject mislabeled, incomplete, or questionable tubes rather than risk a transfusion error. If you discover a labeling error after leaving the room, do not cross out and rewrite—follow rejection and recollection policy.

Transfusion reaction specimens (post-transfusion blood samples, urine, or other materials per protocol) also route to blood bank or transfusion services with urgent documentation—not to routine chemistry drop boxes.

Central Receiving vs Direct Section Delivery

Many hospitals use central specimen receiving to accession, centrifuge, and sort. In that model you deliver to one location with barcode scanning; the lab routes internally. You still must:

  • Separate temperature classes (frozen, refrigerated, ambient, 37°C warm pack, ice slurry).
  • Bag biohazard specimens with paperwork in the outer pocket.
  • Flag STAT, timed, and add-on specimens per policy.
  • Never hide a blood bank tube inside a routine chemistry bag without explicit process approval.

Smaller clinics and reference-lab draw sites may courier everything to one external lab address—packaging and temperature then matter more than internal hallway routing (covered in Section 9.2).

Multi-Tube Draws: Practical Routing Example

Order: blood cultures, PT/INR, CBC, CMP.

TubeDepartment
Culture bottlesMicrobiology (first priority delivery)
Light-blue citrateCoagulation
Lavender EDTAHematology
SST/goldChemistry (or central processing for spin)

Deliver cultures immediately. Batch remaining tubes by policy, but do not let coagulation or cultures wait behind a routine chemistry run.

Common Delivery Failures

ErrorConsequencePrevention
Wrong departmentDelayed or wrong testMatch order + tube additive
Underfilled citrateRejected coagulationFill to line; second tube if needed
Blood bank mislabelTransfusion riskBedside two-ID label + verification
Cultures delayedFalse negativesSTAT hand delivery
Leaking bagExposure, lost paperworkCap check, absorbent, outer pocket

Exam Scenarios to Rehearse

  • Nurse asks you to drop a lavender and light-blue in the chemistry window "because the lab is closer." Refuse—route by test, not geography.
  • You find an unlabeled pink tube in your carrier. Do not deliver to blood bank; quarantine and investigate.
  • STAT ammonia on ice arrives with routine tubes in the same unrefrigerated bag. Separate and hand-deliver the iced specimen.

Domain II-D rewards collectors who treat delivery as part of the test method. The right stick in the wrong department is still the wrong result.

Test Your Knowledge

A patient has orders for PT/INR and a CBC. Which delivery routing is correct?

A
B
C
D
Test Your Knowledge

Which specimen most likely requires the strictest identification, bedside labeling, and dedicated blood-bank delivery protocol?

A
B
C
D
Test Your Knowledge

After collecting blood culture bottles and several evacuated tubes from one patient, what delivery priority best protects specimen integrity?

A
B
C
D
Test Your Knowledge

A light-blue sodium citrate tube drawn for aPTT arrives at the laboratory underfilled below the marked line. What is the expected outcome?

A
B
C
D