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100+ Free NLN NEX Science Practice Questions

Pass your NLN Nursing Entrance Exam (NEX) - Science Section exam on the first try — instant access, no signup required.

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A balanced diet that supports overall health should emphasize:

A
B
C
D
to track
2026 Statistics

Key Facts: NLN NEX Science Exam

The NLN NEX Science section is a 60-question, 60-minute multiple-choice test covering biology, human anatomy and physiology, chemistry, and health, with no physics; it is one of three NEX subject tests used for nursing program admission.

Sample NLN NEX Science Practice Questions

Try these sample questions to test your NLN NEX Science exam readiness. Each question includes a detailed explanation. Start the interactive quiz above for the full 100+ question experience with AI tutoring.

1Which organelle is the primary site of ATP production in a human cell?
A.Mitochondrion
B.Ribosome
C.Lysosome
D.Golgi apparatus
Explanation: The mitochondrion is the site of aerobic cellular respiration, where the electron transport chain generates the bulk of the cell's ATP. It is often called the powerhouse of the cell for this reason.
2What is the function of the cell membrane (plasma membrane)?
A.Regulating what enters and leaves the cell
B.Storing the cell's genetic material
C.Producing ribosomal RNA
D.Carrying out photosynthesis
Explanation: The plasma membrane is a selectively permeable phospholipid bilayer that controls the passage of substances into and out of the cell. This selective control helps maintain homeostasis within the cell.
3During diffusion, molecules move from an area of:
A.Lower to higher concentration using ATP
B.Higher to lower concentration without energy input
C.Lower to higher concentration without energy input
D.Equal concentrations only
Explanation: Diffusion is the passive movement of molecules from a region of higher concentration to one of lower concentration, down the concentration gradient. Because it follows the gradient, it requires no cellular energy.
4Osmosis specifically refers to the movement of which substance across a semipermeable membrane?
A.Glucose
B.Sodium ions
C.Water
D.Oxygen
Explanation: Osmosis is the diffusion of water across a selectively permeable membrane from a region of lower solute concentration to a region of higher solute concentration. It is a special case of diffusion involving only water.
5A red blood cell placed in a hypotonic solution will most likely:
A.Shrink and crenate
B.Swell and possibly burst
C.Remain unchanged
D.Become more rigid
Explanation: A hypotonic solution has a lower solute concentration than the cell's interior, so water moves into the cell by osmosis. The red blood cell swells and may lyse (burst) if enough water enters.
6Which structure within the nucleus is responsible for assembling ribosomes?
A.Nucleolus
B.Chromatin
C.Nuclear pore
D.Centriole
Explanation: The nucleolus is a dense region within the nucleus where ribosomal RNA is synthesized and ribosomal subunits are assembled. These subunits then exit to the cytoplasm to build ribosomes.
7Which phase of mitosis is characterized by sister chromatids separating and moving toward opposite poles?
A.Prophase
B.Metaphase
C.Anaphase
D.Telophase
Explanation: During anaphase, the centromeres split and the sister chromatids are pulled apart toward opposite poles of the cell by spindle fibers. This ensures each daughter cell receives an identical set of chromosomes.
8How many daughter cells result from a single cell completing meiosis?
A.Two diploid cells
B.Two haploid cells
C.Four diploid cells
D.Four haploid cells
Explanation: Meiosis consists of two consecutive divisions that produce four genetically distinct daughter cells, each with half the chromosome number of the parent (haploid). This process generates gametes for sexual reproduction.
9What is the main difference between mitosis and meiosis?
A.Mitosis produces gametes; meiosis produces body cells
B.Mitosis produces identical diploid cells; meiosis produces genetically varied haploid cells
C.Mitosis occurs only in plants; meiosis occurs only in animals
D.Mitosis requires no DNA replication; meiosis requires two replications
Explanation: Mitosis produces two diploid cells genetically identical to the parent for growth and repair, while meiosis produces four genetically varied haploid gametes. Meiosis introduces variation through crossing over and independent assortment.
10In DNA, the nitrogenous base adenine always pairs with:
A.Guanine
B.Cytosine
C.Thymine
D.Uracil
Explanation: In DNA, complementary base pairing dictates that adenine pairs with thymine via two hydrogen bonds, and guanine pairs with cytosine. This consistent pairing allows accurate DNA replication.

About the NLN NEX Science Exam

The Science section of the NLN Nursing Entrance Exam (NEX) is one of three subject tests, alongside Verbal and Mathematics, used by nursing programs to evaluate applicants' academic readiness. It contains 60 multiple-choice questions (55 scored and 5 unscored pilot items), each with four answer options and a 60-minute time limit. The section measures general science knowledge across four areas: biology, human anatomy and physiology, chemistry, and health, set primarily at the high-school level with some introductory college content. The NEX replaced the older NLN PAX in 2025 and removed physics entirely from the Science section. There is no penalty for guessing, but unanswered items are scored as incorrect, so applicants should answer every question.

Questions

60 scored questions

Time Limit

60 minutes

Passing Score

No universal cut score; the Science test reports percent correct and a percentile rank, and each nursing program sets its own minimum. Nationally, about 69% correct meets the 50th percentile for RN applicants and about 56% for LPN/VN applicants.

Exam Fee

Approximately $73.50 to $80.00 for the full NEX (all three sections); the Science section is not sold separately. (National League for Nursing (NLN))

NLN NEX Science Exam Content Outline

36%

Biology

Cell structure and function, diffusion and osmosis, mitosis and meiosis, genetics and DNA, photosynthesis, cellular respiration, evolution, classification, ecology, microbiology, and laboratory procedures.

20%

Human Anatomy

Directional terms, body planes, organ systems and major organs, and the structure of the cardiovascular, skeletal, muscular, integumentary, nervous, endocrine, respiratory, digestive, urinary, lymphatic, and sensory systems.

20%

Human Physiology

Function of body systems, homeostasis and feedback mechanisms, cellular processes, and the physiology of the cardiovascular, respiratory, endocrine, nervous, digestive, urinary, and immune systems.

9%

Chemistry

Atomic structure, periodic table, physical and chemical change, chemical bonding, acids, bases and pH, solutions, and basic organic chemistry.

15%

Health

Individual and community/population health, substance use, physical health, growth and development, mental and emotional health, nutrition, prevention and safety, and the physical environment.

How to Pass the NLN NEX Science Exam

What You Need to Know

  • Passing score: No universal cut score; the Science test reports percent correct and a percentile rank, and each nursing program sets its own minimum. Nationally, about 69% correct meets the 50th percentile for RN applicants and about 56% for LPN/VN applicants.
  • Exam length: 60 questions
  • Time limit: 60 minutes
  • Exam fee: Approximately $73.50 to $80.00 for the full NEX (all three sections); the Science section is not sold separately.

Keys to Passing

  • Complete 500+ practice questions
  • Score 80%+ consistently before scheduling
  • Focus on highest-weighted sections
  • Use our AI tutor for tough concepts

NLN NEX Science Study Tips from Top Performers

1Focus most of your study time on biology and anatomy and physiology, which together make up about three-quarters of the scored Science items.
2Do not study physics for the NEX; it was removed from the Science section, so spend that time on biology, A and P, chemistry, and health instead.
3Master the levels of biological organization and directional and anatomical terms early, since they anchor many anatomy and physiology questions.
4Review homeostasis and negative and positive feedback loops, as physiology items frequently test how body systems maintain balance.
5Learn high-yield chemistry basics such as atomic structure, the pH scale, bonding, and acid-base reactions, since chemistry is a small but scored area.
6Answer every question because there is no penalty for guessing, and use the 60-minute limit to pace yourself at roughly one minute per item.

Frequently Asked Questions

How many questions are on the NLN NEX Science section and how much time is allowed?

The Science section has 60 multiple-choice questions, of which 55 are scored and 5 are unscored pilot items. You have 60 minutes to complete the section, and each question has four answer options with one correct answer.

Does the NLN NEX Science section include physics?

No. When the NEX replaced the older NLN PAX in 2025, physics was removed entirely from the Science section. The section now covers only biology, human anatomy, human physiology, chemistry, and health.

What topics are covered on the NEX Science section?

The Science section covers biology (about 36% of scored items), human anatomy (20%), human physiology (20%), chemistry (9%), and health (15%), set primarily at the high-school level with some introductory college content.

Is there a passing score for the NEX Science section?

There is no universal passing score. The section reports percent correct and a percentile rank, and each nursing program sets its own minimum. Nationally, about 69% correct meets the 50th percentile for RN applicants and about 56% for LPN/VN applicants.

Is the NEX scored with a penalty for guessing?

No, there is no penalty for guessing. Items are scored as correct or incorrect, but unanswered questions are counted as incorrect, so you should answer every question even if you are unsure.

Who administers the NLN NEX and how is it delivered?

The NEX is administered by the National League for Nursing (NLN). It is a computer-based test delivered with a live proctor either in person or virtually, and the Science section is taken together with the Verbal and Math sections in one sitting.