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200+ Free NLN NEX Practice Questions

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Which organelle is known as the "powerhouse of the cell" because it produces the majority of ATP through cellular respiration?

A
B
C
D
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2026 Statistics

Key Facts: NLN NEX Exam

163

Total Questions

NLN NEX 2026

3 hrs

Total Test Time

60 min × 3 sections

37%

Science Section Weight

NLN NEX blueprint

0–300

Composite Score Scale

Percentile-based

No physics

Key Change from PAX

2025 NEX update

$53–74

Exam Registration Fee

NLN 2026

The NLN NEX (launched 2025, replacing the PAX) gives students 60 minutes per section — up from 40 minutes on the PAX. The Science section (37%) is the most content-heavy, covering biology (14%), anatomy (8%), physiology (8%), chemistry (3%), and health (6%). The Verbal section (36%) tests word knowledge and reading comprehension. Mathematics (28%) covers numbers/operations, measurement, algebra, and data — with an on-screen calculator available. Scores are reported as a composite 0–300 based on percentile ranks from LPN/VN and RN norming groups.

Sample NLN NEX Practice Questions

Try these sample questions to test your NLN NEX exam readiness. Each question includes a detailed explanation. Start the interactive quiz above for the full 200+ question experience with AI tutoring.

1Which organelle is known as the "powerhouse of the cell" because it produces the majority of ATP through cellular respiration?
A.Mitochondrion
B.Ribosome
C.Golgi apparatus
D.Endoplasmic reticulum
Explanation: Mitochondria carry out aerobic respiration, producing up to 36–38 ATP molecules per glucose molecule through the Krebs cycle and oxidative phosphorylation. The ribosome makes proteins, the Golgi apparatus processes and packages proteins, and the endoplasmic reticulum is involved in protein and lipid synthesis.
2A student observes a cell under a microscope and notes that it has a cell wall, chloroplasts, and a large central vacuole. This cell is most likely from which organism?
A.A plant
B.A bacterium
C.An animal
D.A fungus
Explanation: Plant cells are uniquely characterized by a rigid cell wall (made of cellulose), chloroplasts for photosynthesis, and a large central vacuole for turgor pressure and storage. Animal cells lack all three features. Bacteria have a cell wall but no chloroplasts or central vacuole, and fungal cell walls are made of chitin, not cellulose.
3During which phase of mitosis do chromosomes line up along the cell's equatorial plate?
A.Metaphase
B.Prophase
C.Anaphase
D.Telophase
Explanation: In metaphase, replicated chromosomes (each consisting of two sister chromatids) are aligned at the metaphase plate (the cell's equator) by spindle fibers. Prophase is when chromosomes condense; anaphase is when sister chromatids are pulled apart; telophase is when nuclear envelopes reform around the separated chromosome sets.
4A nitrogenous base in DNA pairs with thymine (T). Which base is it paired with?
A.Adenine (A)
B.Guanine (G)
C.Cytosine (C)
D.Uracil (U)
Explanation: In DNA, adenine (A) pairs with thymine (T) via two hydrogen bonds, and guanine (G) pairs with cytosine (C) via three hydrogen bonds. Uracil replaces thymine in RNA but is not present in DNA. This complementary base pairing is the foundation of DNA replication and transcription.
5Which type of RNA carries the amino acid to the ribosome during protein synthesis?
A.Transfer RNA (tRNA)
B.Messenger RNA (mRNA)
C.Ribosomal RNA (rRNA)
D.Small nuclear RNA (snRNA)
Explanation: Transfer RNA (tRNA) acts as an adapter: it carries a specific amino acid on one end and has an anticodon on the other end that matches the codon on mRNA. mRNA carries the genetic code from the nucleus to the ribosome; rRNA is a structural component of the ribosome itself; snRNA is involved in splicing pre-mRNA.
6In a monohybrid cross between two pea plants that are both heterozygous for seed color (Yy × Yy, where Y = yellow is dominant), what percentage of offspring will show the recessive phenotype?
A.25%
B.50%
C.75%
D.100%
Explanation: A Yy × Yy cross produces offspring in a 1:2:1 ratio (YY : Yy : yy). Only the yy genotype expresses the recessive phenotype (green), which is 1 out of 4 combinations, or 25%. The remaining 75% (YY + Yy) show the dominant yellow phenotype. This 3:1 phenotypic ratio is a hallmark of Mendel's Law of Segregation.
7What is the term for the movement of water molecules across a semipermeable membrane from an area of higher water concentration to an area of lower water concentration?
A.Osmosis
B.Active transport
C.Facilitated diffusion
D.Endocytosis
Explanation: Osmosis is the passive movement of water across a semipermeable membrane down its concentration gradient (from high water concentration—hypotonic solution—toward low water concentration—hypertonic solution). Active transport requires energy (ATP). Facilitated diffusion uses carrier proteins but moves solutes, not water. Endocytosis engulfs large particles by membrane folding.
8A red blood cell is placed in a very salty (hypertonic) solution. What will happen to the cell?
A.It will shrink (crenate) as water leaves the cell
B.It will swell and eventually burst (lyse)
C.It will remain unchanged in size
D.It will divide by mitosis
Explanation: In a hypertonic solution, the solute concentration outside the cell is higher than inside. By osmosis, water moves out of the cell toward the higher solute concentration, causing the cell to shrink or crenate. A cell placed in a hypotonic solution would swell because water enters the cell. An isotonic solution causes no net water movement.
9Which level of biological organization includes all organisms of the same species living in a specific area at the same time?
A.Population
B.Community
C.Ecosystem
D.Biome
Explanation: A population consists of all individuals of the same species within a defined area at a given time. A community includes all populations of different species living together in an area. An ecosystem adds the abiotic (nonliving) components. A biome is a large geographic region characterized by climate and the types of organisms that live there.
10During aerobic cellular respiration, which stage produces the most ATP?
A.Oxidative phosphorylation (electron transport chain)
B.Glycolysis
C.Krebs cycle
D.Fermentation
Explanation: Oxidative phosphorylation via the electron transport chain produces approximately 32–34 ATP molecules per glucose, by far the most of any stage. Glycolysis nets only 2 ATP; the Krebs cycle also yields 2 ATP per glucose (but produces NADH/FADH₂ for the ETC). Fermentation is anaerobic and produces only 2 ATP net.

About the NLN NEX Exam

The NLN NEX (Nursing Entrance Exam) replaced the PAX in 2025. It tests academic readiness for nursing school across three sections: Verbal Ability (58 questions, 36%), Mathematics (45 questions, 28%), and Science (60 questions, 37%). No physics — the science section focuses on biology, anatomy, physiology, chemistry, and health.

Questions

163 scored questions

Time Limit

3 hours (60 min per section)

Passing Score

Program-dependent (50th+ percentile typical)

Exam Fee

$53-74 (National League for Nursing (NLN))

NLN NEX Exam Content Outline

37%

Science (60 questions)

Biology (14%), Human Anatomy (8%), Human Physiology (8%), Chemistry (3%), Health & Nutrition (6%) — no physics

36%

Verbal Ability (58 questions)

Word Knowledge (17%): vocabulary in context; Reading Comprehension (17%): main idea, inference, and author's purpose

28%

Mathematics (45 questions)

Numbers & Operations (12 questions), Measurement & Conversions (14 questions), Algebra (7 questions), Data & Information (7 questions)

How to Pass the NLN NEX Exam

What You Need to Know

  • Passing score: Program-dependent (50th+ percentile typical)
  • Exam length: 163 questions
  • Time limit: 3 hours (60 min per section)
  • Exam fee: $53-74

Keys to Passing

  • Complete 500+ practice questions
  • Score 80%+ consistently before scheduling
  • Focus on highest-weighted sections
  • Use our AI tutor for tough concepts

NLN NEX Study Tips from Top Performers

1Science is 37% of the exam — prioritize biology, anatomy, and physiology heavily
2No physics on the NEX — skip physics study materials and focus on health science content
3Use the on-screen calculator for math, but practice setting up problems correctly first
4Build vocabulary through root words, prefixes, and suffixes — many NEX words are medical
5For reading comprehension, practice answering only from what the passage states — avoid outside knowledge
6Time yourself: 60 minutes per 58–60 questions means about 1 minute per question

Frequently Asked Questions

What replaced the NLN PAX exam?

The NLN Nursing Entrance Exam (NEX) replaced the PAX in 2025. Key changes include 60 minutes per section (vs. 40 for PAX), removal of all physics content, addition of an on-screen calculator for the math section, and composite scoring on a 0–300 scale based on percentile ranks from norming groups.

What science topics are on the NLN NEX?

The NEX Science section (60 questions) covers Biology (20 scored items), Human Anatomy (11 items), Human Physiology (11 items), Chemistry (5 items), and Health (8 items). Physics was completely removed from the NEX — this is a major change from the old PAX.

Is there a calculator on the NLN NEX?

Yes — an on-screen basic calculator is provided for the Mathematics section on the NEX. This is new compared to the old PAX, which did not allow a calculator. However, you still need to understand how to set up problems correctly.

What score do I need to pass the NLN NEX?

There is no universal passing score. Each nursing program sets its own minimum requirements. Many programs expect a composite score at or above the 50th percentile for their target comparison group (LPN/VN or RN norms). Check your specific program's admissions requirements.

How is the NLN NEX scored?

The NEX uses percentile-based composite scoring (0–300 scale). Your raw score in each section is converted to a percentile rank using either the LPN/VN or RN norming group. The three percentile ranks are summed to create a composite score. A composite of 150 means you performed at the 50th percentile in all three sections.

How should I prepare for the NLN NEX?

Focus most study time on Science (37% of the exam) — especially anatomy, physiology, and biology. Review math fundamentals including fractions, percentages, unit conversions, and basic algebra. For Verbal, practice reading comprehension with health-related passages and build vocabulary using root words and context clues. Plan 6–8 weeks of preparation with 1–2 hours of daily practice.