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Exam Review

Key Facts: BSEH SS Biotechnology Exam

2½ hours

Typical BSEH Senior Secondary Biotechnology theory exam duration

BSEH Class 12 Biotechnology syllabus (code 871)

60+20+20

Theory + practical + internal assessment mark split (grand total 100)

BSEH Class 12 Biotechnology syllabus 2025-26

Code 871

Biotechnology subject code on BSEH Senior Secondary materials

BSEH syllabus / date sheet

English MCQ adaptation

This free local bank is not the official Senior Secondary paper format

OpenExamPrep practice policy

BSEH Senior Secondary Biotechnology is a Class 12 public-exam elective with about 2½ hours of theory (60 marks, date-sheet code 871) plus 20 practical and 20 internal (total 100), covering rDNA, proteins, genomics, and cell culture — not a pure MCQ board paper. This free 2026 bank is an English MCQ study adaptation for CBSE/BSEH-focused Class 12 Biotechnology concepts.

Sample BSEH SS Biotechnology Practice Questions

Try these sample questions to review concepts for the BSEH SS Biotechnology exam. Each question includes a detailed explanation. Start the interactive quiz above for the full 100+ question experience with AI tutoring.

1Restriction endonucleases used in rDNA technology typically cut DNA at:
A.Random single-stranded nicks only
B.Specific recognition sequences (restriction sites)
C.Only RNA–DNA hybrids
D.Telomeres exclusively
Explanation: Type II restriction endonucleases recognise short palindromic DNA sequences and cleave within or near those sites, producing sticky or blunt ends useful for cloning.
2Which enzyme seals nicks to join DNA fragments during cloning?
A.DNA ligase
B.DNA polymerase I only
C.Reverse transcriptase
D.Helicase
Explanation: DNA ligase catalyses phosphodiester bond formation between adjacent 5′-phosphate and 3′-OH ends, sealing sticky or blunt-end joins after restriction digestion.
3RFLP analysis distinguishes alleles primarily by:
A.Differences in restriction fragment lengths after digestion
B.Protein isoelectric points only
C.mRNA poly-A tail length
D.Ribosome binding rates
Explanation: Restriction Fragment Length Polymorphism detects sequence differences that create or destroy restriction sites (or change fragment sizes), yielding different band patterns after electrophoresis.
4A plasmid cloning vector typically requires all of the following EXCEPT:
A.An origin of replication
B.A selectable marker (e.g. antibiotic resistance)
C.A multiple cloning site
D.A complete eukaryotic centromere
Explanation: Bacterial plasmids need ori, selectable markers, and MCS for inserts; a eukaryotic centromere is not required for standard bacterial plasmid vectors (YACs need centromeres).
5Cosmids are hybrid vectors that combine features of:
A.Plasmids and λ phage cos sites
B.YAC and BAC only
C.Ti plasmid and animal viruses only
D.mRNA and tRNA
Explanation: Cosmids carry plasmid ori/selectable markers plus λ cos sites, allowing packaging of large inserts (~30–45 kb) into phage particles for efficient delivery.
6YACs (yeast artificial chromosomes) are preferred when the insert size needed is:
A.Very large genomic fragments (hundreds of kb)
B.Only short oligonucleotides (<20 bp)
C.Single amino acids
D.Lipid bilayers
Explanation: YACs can maintain megabase-scale inserts in yeast, enabling large-genome cloning that exceeds typical plasmid capacity.
7BAC vectors are based on the F plasmid of E. coli and typically accept inserts of roughly:
A.~100–350 kb
B.Only 5–10 bp
C.Entire eukaryotic chromosomes always
D.RNA genomes exclusively
Explanation: Bacterial artificial chromosomes stably maintain large inserts (order of 100+ kb) at low copy number, useful for genome libraries.
8Introduction of naked DNA into bacterial cells is called:
A.Transformation
B.Transcription
C.Transduction only
D.Translation
Explanation: Transformation is uptake of free DNA by competent bacterial cells. Transfection often refers to eukaryotic cells; transduction uses phage.
9Electroporation introduces DNA into cells by:
A.Brief high-voltage pulses that permeabilise membranes
B.Heating DNA to 100 °C only
C.Digesting the cell wall with trypsin exclusively
D.Blocking all ion channels permanently
Explanation: Electroporation uses electrical pulses to create transient pores in membranes, allowing DNA entry into bacteria, plant protoplasts, or animal cells.
10Biolistics (gene gun) delivers DNA primarily by:
A.High-velocity microprojectiles coated with DNA
B.Only conjugation pili
C.Passive diffusion through nuclear pores without force
D.Antibody-mediated endocytosis only
Explanation: Biolistics accelerates gold/tungsten particles coated with DNA into plant (or other) cells—useful when Agrobacterium methods are difficult.

About the BSEH SS Biotechnology Exam

Haryana Senior Secondary Biotechnology under BSEH covers Class 12 Bio-Technology aligned to the Board’s Senior Secondary syllabus and the CBSE Class XII Biotechnology textbook. Core topics include recombinant DNA technology, protein structure and engineering, genomics and bioinformatics, microbial cell culture, plant cell culture and transgenic applications, and animal cell culture with hybridoma and therapeutic protein production. Official assessment is a theory paper of about 2½ hours for 60 marks (date-sheet code 871), with 20 practical and 20 internal assessment marks. This free local bank provides English four-option MCQs for revision; pair it with official syllabus PDFs, practical work, and written answers via bseh.org.in.

Exam sponsor: Board of School Education Haryana (BSEH). The requirements and fees below concern the certification or admission exam, separate from our free practice resources.

Assessment

BSEH Senior Secondary Biotechnology is a Class 12 theory elective under the Senior Secondary Certificate Examination (date-sheet code 871). Official syllabus logistics describe about 2½ hours of theory work for 60 marks, plus practical examination (20) and internal assessment (20) for a grand total of 100. Theory is split into two 30-mark units: Protein and Gene Manipulation (recombinant DNA technology; protein structure and engineering; genomics and bioinformatics) and Cell Culture and Genetic Manipulation (microbial, plant, and animal cell culture and applications). Hindi/English medium materials are typical; this practice bank is an English-language MCQ study adaptation. Confirm the current syllabus PDF and circulars on bseh.org.in.

Time Limit

2½ hours

Passing Score

33%

Exam / Certification Fees

As notified by BSEH and paid through affiliated schools for regular and compartment/improvement sittings (no single fixed public subject fee for all categories).

Exam sponsor website

Fees, eligibility, and exam policies can change. Confirm them with the exam sponsor before applying or paying.

Our practice resources: topics covered

We aim to reflect publicly available exam outlines and topic information in our study resources. Coverage, format, and difficulty may differ from the actual exam, and we cannot guarantee that every detail is accurate or current. Confirm exam requirements, fees, and policies with the official exam sponsor.

~20% of this local bank

Recombinant DNA Technology

Enzymes, vectors, host methods, PCR, hybridisation, libraries, sequencing, and mutagenesis.

~15% of this local bank

Protein Structure and Engineering

3-D structure, structure–function, purification, therapeutic proteins, and engineering examples.

~15% of this local bank

Genomics and Bioinformatics

Genome projects, SNPs, microarrays, proteomics, NCBI, and BLAST.

~17% of this local bank

Microbial Cell Culture

Culture types, growth kinetics, scale-up, strain improvement, applications, and biosafety.

~17% of this local bank

Plant Cell Culture

Tissue-culture methods, regeneration, transgenic traits, metabolites, and plant biosafety.

~16% of this local bank

Animal Cell Culture

Cell lines, culture environment, therapeutics, hybridoma/mAbs, stem cells, and tissue engineering.

Preparing for the BSEH SS Biotechnology Exam

What You Need to Know

  • Passing score: 33%
  • Assessment: BSEH Senior Secondary Biotechnology is a Class 12 theory elective under the Senior Secondary Certificate Examination (date-sheet code 871). Official syllabus logistics describe about 2½ hours of theory work for 60 marks, plus practical examination (20) and internal assessment (20) for a grand total of 100. Theory is split into two 30-mark units: Protein and Gene Manipulation (recombinant DNA technology; protein structure and engineering; genomics and bioinformatics) and Cell Culture and Genetic Manipulation (microbial, plant, and animal cell culture and applications). Hindi/English medium materials are typical; this practice bank is an English-language MCQ study adaptation. Confirm the current syllabus PDF and circulars on bseh.org.in.
  • Time limit: 2½ hours
  • Exam / certification fees: As notified by BSEH and paid through affiliated schools for regular and compartment/improvement sittings (no single fixed public subject fee for all categories). Official sources

Using Our Practice Resources

  • Work through all 100 available questions
  • Review every answer and explanation
  • Track weak areas and revisit them
  • Use our AI tutor for tough concepts

BSEH SS Biotechnology: Suggested Study Strategy

1Build a one-page map of rDNA workflow: enzyme → vector → host → selection → PCR/hybridisation → sequencing — then drill each step with MCQs.
2For culture questions, always note organism type (microbe vs plant vs animal) before choosing media, vessels, or gene-transfer methods.
3Memorise hallmark products: Bt crops, edible vaccines, tPA, EPO, Factor VIII/IX, OKT3, Herceptin — common objective targets.
4Link structure to function: sickle-cell Glu→Val and chymotrypsin catalytic triad appear often in protein chapters.
5Use current BSEH Class 12 Biotechnology syllabus and date-sheet materials from bseh.org.in to balance theory revision with the prescribed practical list.

Frequently Asked Questions

How is BSEH Senior Secondary Biotechnology officially examined?

Biotechnology is examined under the Haryana Senior Secondary Certificate Examination as a Class 12 elective. Official syllabus materials list Biotechnology as code 871 with about 2½ hours of theory (60 marks), practical examination (20 marks), and internal assessment (20 marks). Confirm the year’s syllabus PDF, date sheet, and circulars on bseh.org.in.

Is this practice bank the same format as the official Senior Secondary Biotechnology paper?

No. Official BSEH Class 12 Biotechnology theory papers mix objective and subjective sections (MCQ/fill-in/assertion-reason, very short, short, and essay). This bank is an English-language multiple-choice study adaptation for concepts only — not an official translation, blueprint replica, or full written-paper simulation.

What syllabus does this bank follow?

It is aligned to BSEH Senior Secondary (Class 12) Bio-Technology / CBSE Class XII Biotechnology style scope: recombinant DNA technology, protein structure and engineering, genomics and bioinformatics, microbial cell culture, plant cell culture and applications, and animal cell culture and applications (including biosafety themes).

What is the pass mark for BSEH Senior Secondary Biotechnology?

BSEH Senior Secondary subject pass floor is commonly stated as 33% for theory electives. Always verify the current BSEH Senior Secondary notification for exact criteria for your sitting, including practical components where applicable.

Where should I check official syllabus and date sheets?

Use the Board of School Education Haryana website at bseh.org.in for Class 12 syllabus catalog entries (including Biotechnology code 871), date sheets, and exam circulars. The subject syllabus PDF is published under the Class 12 syllabus materials.