3.4 Infection Control and Safety

Key Takeaways

  • The chain of infection has six links: infectious agent, reservoir, portal of exit, mode of transmission, portal of entry, and susceptible host — breaking any link prevents infection
  • Standard precautions apply to ALL patients regardless of diagnosis and include hand hygiene, PPE use, respiratory hygiene, and safe injection practices
  • Transmission-based precautions include contact (gowns, gloves), droplet (surgical mask), and airborne (N95 respirator, negative pressure room)
  • Hand hygiene is the single most effective method of infection prevention — wash with soap and water for 20+ seconds or use alcohol-based hand sanitizer
  • The three levels of infection control are sanitization (cleaning), disinfection (intermediate), and sterilization (highest — kills all microorganisms including spores)
  • Bloodborne pathogen exposure requires immediate wound care, reporting, and post-exposure prophylaxis evaluation per OSHA standards
Last updated: March 2026

Infection Control and Safety

Infection control is a critical responsibility for every medical assistant. With 15 items on the CCMA exam, this sub-area covers the principles, practices, and regulations that prevent the spread of infection in healthcare settings.


The Chain of Infection

Infection requires all six links in the chain. Breaking any link prevents the spread of infection:

LinkDescriptionExampleHow to Break It
1. Infectious agentThe pathogen (bacteria, virus, fungus, parasite)MRSA, influenza, C. difficileSterilization, disinfection, antibiotics
2. ReservoirWhere the pathogen lives and multipliesHumans, animals, contaminated equipmentCleaning, proper waste disposal
3. Portal of exitHow the pathogen leaves the reservoirRespiratory secretions, blood, urine, fecesStandard precautions, containment
4. Mode of transmissionHow the pathogen travels to a new hostDirect contact, droplet, airborne, vehicleHand hygiene, PPE, isolation
5. Portal of entryHow the pathogen enters a new hostMucous membranes, broken skin, respiratory tractWound care, PPE, barrier devices
6. Susceptible hostPerson who can become infectedImmunocompromised, elderly, infantsVaccination, nutrition, health maintenance

Types of Microorganisms:

TypeExamplesTreatment
BacteriaMRSA, E. coli, Streptococcus, TBAntibiotics
VirusesInfluenza, HIV, hepatitis, COVID-19Antivirals (limited); prevention via vaccination
FungiCandida (yeast), ringworm, athlete's footAntifungals
ParasitesGiardia, tapeworm, malariaAntiparasitic medications
PrionsCreutzfeldt-Jakob disease (CJD)No treatment; requires special sterilization

Standard Precautions

Standard precautions apply to ALL patients, ALL the time, regardless of known or suspected infection status:

ComponentApplication
Hand hygieneBefore/after patient contact, before/after procedures, after glove removal
PPEGloves, gowns, masks, eye protection — based on anticipated exposure
Respiratory hygiene/cough etiquetteCover coughs, provide tissues, offer masks to symptomatic patients
Safe injection practicesUse new needle and syringe for each injection; single-dose vials preferred
Sharps safetyNever recap needles; dispose in sharps containers immediately
Environmental cleaningClean and disinfect surfaces and equipment between patients
Linen handlingHandle soiled linen minimally; bag at point of use
Patient placementPrivate room for patients who cannot maintain hygiene

Transmission-Based Precautions

Used IN ADDITION TO standard precautions for specific modes of transmission:

TypePPE RequiredRoomExamples
ContactGloves + gownPrivate room preferredMRSA, VRE, C. difficile, scabies, lice
DropletSurgical mask within 3-6 feetPrivate room; door may be openInfluenza, pertussis, meningococcal meningitis, mumps
AirborneN95 respirator (fit-tested)Negative pressure room; door closedTB, measles, chickenpox (varicella), COVID-19 (aerosolizing procedures)

Hand Hygiene

The #1 most effective method of infection prevention:

Handwashing with Soap and Water (20+ seconds):

  1. Wet hands with warm water
  2. Apply soap
  3. Rub palms together, then between fingers, backs of hands, fingertips, thumbs, and wrists
  4. Wash for at least 20 seconds (sing "Happy Birthday" twice)
  5. Rinse thoroughly under running water
  6. Dry with paper towel
  7. Use paper towel to turn off faucet

When to use soap and water (NOT hand sanitizer):

  • Hands are visibly soiled or contaminated
  • Before and after eating
  • After using the restroom
  • After caring for patients with C. difficile or norovirus
  • After contact with blood or body fluids

Alcohol-Based Hand Sanitizer (ABHS):

  • Apply a dime-sized amount to palm
  • Rub all surfaces of hands until dry (~20 seconds)
  • Use when hands are NOT visibly soiled
  • Must be at least 60% alcohol

Personal Protective Equipment (PPE)

PPE Donning Order (putting ON):

  1. Gown — Tie at neck and waist
  2. Mask/respirator — Secure over nose and mouth
  3. Eye protection (goggles or face shield) — Adjust to fit
  4. Gloves — Pull over gown cuffs

PPE Doffing Order (taking OFF):

  1. Gloves — Remove carefully, touching only outside surfaces; dispose
  2. Eye protection — Remove by handle/earpieces (do not touch front)
  3. Gown — Untie and pull away from body, rolling contaminated side inward
  4. Mask/respirator — Remove by ties/elastic (do not touch front)
  5. Perform hand hygiene immediately

Memory aid for doffing order: "Gloves, Goggles, Gown, Mask" — the most contaminated items come off first.


Sanitization, Disinfection, and Sterilization

LevelKillsMethodExamples
SanitizationReduces number of microorganismsWashing with soap and waterPre-cleaning instruments before sterilization
Low-level disinfectionMost bacteria, some viruses, some fungiChemical germicidesStethoscopes, BP cuffs, countertops
Intermediate disinfectionMost bacteria, viruses, fungi (NOT spores)EPA-registered hospital disinfectantBlood spill cleanup
High-level disinfectionAll organisms EXCEPT some sporesChemical sterilants (glutaraldehyde)Semi-critical items (endoscopes)
SterilizationALL organisms INCLUDING sporesAutoclave (steam under pressure)Surgical instruments, needles

Autoclave (Steam Sterilization):

  • Standard cycle: 250°F (121°C) at 15 psi for 15-20 minutes (unwrapped) or 30 minutes (wrapped)
  • Biological indicator (spore test) is the gold standard for verifying sterilization effectiveness
  • Chemical indicators (autoclave tape, strips) show exposure to sterilization conditions but do not confirm sterilization
  • Instruments must be sanitized (cleaned) BEFORE sterilization

OSHA Bloodborne Pathogen Standard

Key Bloodborne Pathogens:

  • Hepatitis B (HBV) — Most resilient; vaccine available; survives on surfaces up to 7 days
  • Hepatitis C (HCV) — Most common chronic bloodborne infection; no vaccine
  • HIV — Least resilient outside the body; no vaccine; manageable with antiretroviral therapy

OSHA Requirements:

  • Written Exposure Control Plan — updated annually
  • Hepatitis B vaccination offered at no cost to employees with occupational exposure
  • Post-exposure evaluation and follow-up for needlestick and exposure incidents
  • Engineering controls — sharps containers, safety needles, self-sheathing devices
  • Work practice controls — no eating/drinking in clinical areas, no recapping needles
  • PPE provided at no cost to employees
  • Annual bloodborne pathogen training for all employees with potential exposure

Needlestick/Exposure Incident Protocol:

  1. Immediately wash the area with soap and water (mucous membranes: flush with water)
  2. Report the incident to your supervisor
  3. Document the incident (source patient, circumstances, body fluid involved)
  4. Seek medical evaluation — source patient testing, baseline labs, PEP consideration
  5. Follow up as directed by occupational health

Biohazardous Waste Disposal

Waste TypeContainerExamples
SharpsPuncture-resistant sharps container (red or labeled)Needles, scalpel blades, lancets, broken glass
BiohazardousRed biohazard bagBlood-soaked gauze, PPE with body fluids, cultures
Regulated medical wasteRed biohazard containerPathological waste, contaminated sharps, cultures
General medical wasteRegular trashPaper towels (no blood), packaging, food waste
Pharmaceutical wasteDesignated containerExpired or unused medications

Never recap needles, bend needles, or remove needles from syringes by hand.

Test Your Knowledge

What is the SINGLE most effective method of preventing the spread of infection in healthcare settings?

A
B
C
D
Test Your Knowledge

A patient with active tuberculosis (TB) requires which type of precautions?

A
B
C
D
Test Your Knowledge

What is the correct order for REMOVING (doffing) PPE?

A
B
C
D
Test Your Knowledge

The autoclave sterilizes instruments using:

A
B
C
D
Test Your KnowledgeOrdering

Arrange the steps of the needlestick/exposure incident protocol in the correct order.

Arrange the items in the correct order

1
Follow up as directed by occupational health
2
Immediately wash the area with soap and water
3
Report the incident to your supervisor
4
Seek medical evaluation (baseline labs, PEP consideration)
5
Document the incident details
Test Your Knowledge

Which of the following bloodborne pathogens can survive on environmental surfaces for up to 7 days?

A
B
C
D
Test Your Knowledge

Which type of transmission-based precautions requires a negative pressure room?

A
B
C
D
Test Your KnowledgeOrdering

Arrange the PPE donning (putting ON) steps in the correct order.

Arrange the items in the correct order

1
Gloves (pull over gown cuffs)
2
Gown (tie at neck and waist)
3
Eye protection (goggles or face shield)
4
Mask or respirator (secure over nose and mouth)