100+ Free ABMM Practice Questions
Pass your Diplomate, American Board of Medical Microbiology (D(ABMM)) exam on the first try — instant access, no signup required.
A Gram-positive coccus in clusters from a wound culture is catalase-positive and coagulase-positive. Which species is most likely?
Key Facts: ABMM Exam
200
Total MCQ Items
Single-best-answer format
6 hrs
Total Exam Time
Single-session computer-based test
25%
Bacteriology Weight
Largest single content domain
$450 / $575
Application Fee
ASM member / non-member
12 states
CLIA Director Recognition
Direct CLIA recognition for high-complexity labs
3 yrs
Recertification Cycle
Continuing Maintenance Program
The ABMM exam (Diplomate, American Board of Medical Microbiology) is a 6-hour, 200-question single-best-answer computer-based test administered by ASM. Application is $450 (member) / $575 (non-member); a separate $400 exam registration fee applies if approved. Passing confers D(ABMM) — recognized under CLIA '88 for high-complexity clinical laboratory director qualification in 12 states. Recertification via the Continuing Maintenance Program every 3 years. Distinct from ABPath Medical Microbiology (MD-only) and ASCP M (BS-level technologist).
Sample ABMM Practice Questions
Try these sample questions to test your ABMM exam readiness. Each question includes a detailed explanation. Start the interactive quiz above for the full 100+ question experience with AI tutoring.
1A Gram-positive coccus in clusters from a wound culture is catalase-positive and coagulase-positive. Which species is most likely?
2A coagulase-negative staphylococcus from a clean-catch urine in a young woman with cystitis is novobiocin-resistant. Which species is most likely?
3A blood culture isolate is a Gram-positive coccus in clusters, coagulase-negative, but ornithine decarboxylase positive and PYR positive. Which species should be suspected?
4A throat culture grows beta-hemolytic colonies that are bacitracin-susceptible and PYR-positive. The most likely organism is:
5A vaginal-rectal swab from a pregnant woman at 36 weeks grows beta-hemolytic colonies that are CAMP-positive and hippurate hydrolysis positive. Which Lancefield group does this organism belong to?
6An alpha-hemolytic Gram-positive coccus from blood is optochin-susceptible and bile-soluble. The organism is:
7A urinary isolate grows on bile-esculin agar with blackening and grows in 6.5% NaCl broth. PYR is positive. The most likely organism is:
8Which test best distinguishes Enterococcus faecium from Enterococcus faecalis in a clinical lab?
9A urine isolate is a lactose-fermenting, indole-positive, motile Gram-negative rod that produces a green sheen on EMB. The organism is:
10A Gram-negative rod from a urine culture is urease-strongly-positive, swarms on blood agar, indole-negative, and produces H2S on TSI. The likely organism is:
About the ABMM Exam
The Diplomate, American Board of Medical Microbiology (D(ABMM)) certification validates doctoral-level expertise to direct a clinical microbiology laboratory. The 6-hour computer-based exam has 200 single-best-answer multiple-choice questions covering bacteriology, antimicrobial susceptibility testing, mycology, mycobacteriology, parasitology, virology, molecular diagnostics, and laboratory administration. Eligibility requires a doctorate (PhD, MD, DO, DVM, DDS/DMD, DrPH) plus 3 years post-doc experience, a 2-year CPEP postdoctoral fellowship, or an ACGME-accredited Medical Microbiology fellowship. CLIA recognizes D(ABMM) for high-complexity lab director qualification in 12 states.
Questions
200 scored questions
Time Limit
6 hours computer-based test (single session)
Passing Score
Criterion-referenced (modified Angoff); average item difficulty target ~0.70
Exam Fee
$450 (ASM member) / $575 (non-member) application + $400 exam registration if approved (American Society for Microbiology (ASM) — American Board of Medical Microbiology)
ABMM Exam Content Outline
Bacteriology
Gram-positive cocci (Staph, Strep, Enterococcus), Enterobacterales, non-fermenters (Pseudomonas, Acinetobacter, Burkholderia, Stenotrophomonas), Neisseria/Moraxella, anaerobes, fastidious organisms (Haemophilus, Legionella, Bordetella, Brucella, Francisella, Bartonella), spirochetes (Treponema, Borrelia, Leptospira), Mycoplasma/Ureaplasma, Chlamydia, Rickettsia/Ehrlichia/Anaplasma/Coxiella. MALDI-TOF and 16S rRNA workflows.
Antimicrobial Susceptibility Testing
CLSI M100 (current edition), broth microdilution, disk diffusion, gradient strip (Etest), automated systems, MIC interpretation and expert rules. ESBL (TEM/SHV/CTX-M), AmpC, carbapenemases (KPC, NDM, OXA-48-like, VIM, IMP), mecA/MRSA cefoxitin, vanA/vanB, MEF/ERM, qnr. mCIM/eCIM and CarbaNP confirmatory tests.
Mycology
Yeasts including Candida auris, Cryptococcus neoformans/gattii, Trichosporon. Dimorphic fungi (Histoplasma, Blastomyces, Coccidioides, Paracoccidioides, Sporothrix, Talaromyces marneffei). Hyaline molds (Aspergillus, Fusarium, Scedosporium/Lomentospora), Mucorales, dematiaceous, dermatophytes, Pneumocystis jirovecii. CLSI M27/M38/M60 antifungal AST, galactomannan, β-D-glucan.
Mycobacteriology
MTB complex, NTM (MAC, M. abscessus, M. kansasii, M. marinum, M. fortuitum), AFB stains, MGIT/Lowenstein-Jensen, MALDI-TOF mycobacteria, line probe assays, Xpert MTB/RIF and MTB/RIF Ultra, CLSI M24 AST, BSL-3 biosafety, IGRA vs TST.
Parasitology
Protozoa (Giardia, Cryptosporidium, Cyclospora, Entamoeba, Plasmodium, Babesia, Trypanosoma, Leishmania, Toxoplasma, Naegleria, Acanthamoeba), helminths (Ascaris, Strongyloides, hookworm, Enterobius, Trichuris, Schistosoma, Taenia, Echinococcus, Diphyllobothrium). Stool O&P, Giemsa thick/thin smears, modified acid-fast, multiplex GI panels.
Virology
Respiratory viruses (Influenza A/B, RSV, hMPV, parainfluenza, adenovirus, rhinovirus, SARS-CoV-2), GI viruses, herpes (HSV, VZV, CMV, EBV, HHV-6/7/8), hepatitis A-E, HIV diagnostic algorithm, HPV, arboviruses (West Nile, Zika, Dengue, chikungunya, EEE/WEE), VHF (BSL-4: Ebola, Lassa, Marburg).
Molecular Diagnostics
PCR, real-time PCR, multiplex syndromic panels (BioFire, GenMark ePlex, NxTAG), NGS, MALDI-TOF, NAATs, isothermal amplification (LAMP, TMA, NEAR), 16S rRNA / ITS sequencing, FDA-cleared vs LDT regulations, validation/verification studies.
Laboratory Administration & Consultation
CLIA '88 high-complexity testing, CAP and ISO 15189 accreditation, biosafety BSL-1 through BSL-4, HHS/USDA select agents, QA/QC and Westgard rules, proficiency testing, IQCP, LIS, billing/CPT, antimicrobial stewardship consultation, cumulative antibiograms, public health reporting.
How to Pass the ABMM Exam
What You Need to Know
- Passing score: Criterion-referenced (modified Angoff); average item difficulty target ~0.70
- Exam length: 200 questions
- Time limit: 6 hours computer-based test (single session)
- Exam fee: $450 (ASM member) / $575 (non-member) application + $400 exam registration if approved
Keys to Passing
- Complete 500+ practice questions
- Score 80%+ consistently before scheduling
- Focus on highest-weighted sections
- Use our AI tutor for tough concepts
ABMM Study Tips from Top Performers
Frequently Asked Questions
What is the D(ABMM) credential?
D(ABMM) — Diplomate of the American Board of Medical Microbiology — is a doctoral-level certification awarded by the American Society for Microbiology (ASM) that validates expertise to direct a clinical microbiology laboratory. CLIA '88 recognizes D(ABMM) as a qualified high-complexity laboratory director in 12 U.S. states. The credential is held primarily by PhDs but is open to MDs, DOs, DVMs, DDS/DMDs, and DrPHs who meet the eligibility pathways.
Who is eligible to take the ABMM exam?
Candidates must hold an earned doctorate (PhD, MD, DO, DVM, DDS/DMD, or DrPH) and meet one of three experience pathways: (1) at least 3 years of post-doctoral experience in medical/clinical microbiology, (2) completion of a 2-year ASM CPEP-accredited postdoctoral fellowship in medical and public health microbiology, or (3) completion of an ACGME-accredited Medical Microbiology fellowship. The ABMM credentialing committee reviews each application before exam registration is permitted.
What is the format of the ABMM exam?
The ABMM exam is a 6-hour computer-based test of 200 single-best-answer multiple-choice questions delivered in a single session. The content blueprint covers Bacteriology (~25%), Virology (~12%), Mycology (~10%), Antimicrobial Susceptibility Testing (~10%), Mycobacteriology (~7%), Parasitology (~7%), Molecular Diagnostics (~15%), and Laboratory Administration (~14%). Pass status is criterion-referenced via the modified Angoff method; the average item difficulty target is approximately 0.70.
How much does the ABMM exam cost in 2026?
The application fee is $450 for ASM members and $575 for non-members, paid at submission and nonrefundable. If the credentialing committee approves the application, candidates pay an additional $400 exam registration fee before scheduling. Total first-attempt cost is approximately $850 (member) or $975 (non-member). Continuing Maintenance Program (CMP) fees apply every 3 years to maintain the credential.
How does ABMM differ from ABPath Medical Microbiology?
ABMM (D(ABMM)) is administered by ASM and is the doctoral-level credential primarily held by PhDs (open to all earned doctorates). ABPath Medical Microbiology is a subspecialty of the American Board of Pathology and is restricted to MDs/DOs who hold ABPath AP/CP or CP primary certification plus a 12-month ACGME Medical Microbiology fellowship. Both qualify the holder to direct a clinical microbiology lab; ABMM is the more common path for non-physician scientists.
Is D(ABMM) recognized by CLIA?
Yes. CLIA '88 explicitly recognizes D(ABMM) certification as qualifying a doctoral-level scientist to serve as a high-complexity clinical laboratory director in 12 states. State-specific licensure (e.g., New York Clinical Laboratory Technology Certificate of Qualification, California Laboratory Field Services) may impose additional requirements on top of D(ABMM).
What are the highest-yield topics?
Bacteriology (~25%) and Molecular Diagnostics (~15%) together cover 40% of the exam — master CLSI M100 breakpoint interpretation, ESBL/AmpC/carbapenemase detection (mCIM, CarbaNP), MRSA cefoxitin screen, vanA/vanB VRE, MALDI-TOF identification, multiplex syndromic panels (BioFire FilmArray), 16S rRNA sequencing applications, and FDA-cleared vs LDT validation. Lab Administration (~14%) is heavily weighted — know CLIA, CAP, ISO 15189, biosafety levels, and antimicrobial stewardship deeply.
How should I prepare for the ABMM exam?
Plan a 6-12 month focused review during or immediately after a CPEP/ACGME fellowship or ABMM-eligible directed practice. Work through the ASM Manual of Clinical Microbiology cover-to-cover, study the current CLSI M100/M24/M27/M38/M60 standards, complete thousands of board-style questions, and take at least two timed full-length 200-question mock exams. The ASM ABMM/CPEP study guide and the ASM Press 'Clinical Microbiology Procedures Handbook' are core references.