Key Takeaways
- A hematoma (bruise) is the most common complication of venipuncture — caused by blood leaking into surrounding tissue from a punctured vein
- Syncope (fainting) is managed by stopping the draw, removing the needle, applying pressure, lowering the patient's head, and applying a cold compress to the forehead or back of the neck
- Nerve injury presents as sharp, shooting, electric-like pain radiating down the arm — IMMEDIATELY stop the draw and remove the needle
- Petechiae (small red dots on the skin) appearing above the tourniquet site indicate a possible platelet disorder or fragile capillaries
- If a venipuncture is unsuccessful after TWO attempts, the phlebotomist must ask another qualified person to attempt the draw
- Hemolysis (rupture of red blood cells) is caused by using a needle that is too small, pulling the syringe plunger too fast, shaking tubes, or forcing blood through a small needle
- An accidental arterial puncture is identified by bright red, pulsating blood — apply firm pressure for at least 5 minutes and do NOT apply a tourniquet
- If a vein collapses during blood draw, try releasing the tourniquet slightly, reducing vacuum (use a smaller tube or syringe), or repositioning the needle slightly
Difficult Draws & Complications
Even experienced phlebotomists encounter difficult draws and complications. Knowing how to prevent, recognize, and manage these situations is essential for patient safety and specimen quality.
Common Complications
1. Hematoma (Most Common Complication)
A hematoma is a bruise caused by blood leaking from the vein into surrounding tissue.
Causes:
- Needle inserted through both walls of the vein
- Needle partially in the vein
- Tourniquet left on too long
- Inadequate pressure applied after needle removal
- Fragile or small veins
Prevention:
- Use proper needle insertion technique (15-30 degree angle)
- Anchor the vein well before puncture
- Apply firm pressure for 3-5 minutes after the draw
- Do not bend the arm after the draw — this does NOT help and can worsen bleeding
Management:
- If a hematoma forms during the draw: immediately remove the needle, apply firm pressure, and elevate the arm
- Apply cold compress for 15-20 minutes to reduce swelling
- Document the occurrence
2. Syncope (Fainting)
Signs of impending syncope:
- Lightheadedness, dizziness
- Pale, cool, clammy skin
- Nausea
- Tunnel vision or loss of vision
Management:
- Stop the draw immediately and remove the needle safely
- Apply pressure to the puncture site
- If seated: lower the patient's head between their knees (or recline the chair)
- If supine: elevate the legs
- Apply cold compress to the forehead or back of the neck
- Loosen tight clothing
- Use ammonia inhalants (smelling salts) if available — wave under the nose, do NOT hold directly against the nostrils
- Never leave a fainting patient unattended
- Document the incident
3. Nerve Injury
Signs:
- Sharp, shooting, electric-like pain radiating down the arm or into the fingers
- Numbness or tingling
- This is DIFFERENT from the normal brief sting of needle insertion
Management:
- IMMEDIATELY remove the needle — do NOT redirect or reposition
- Apply pressure to the site
- Document the incident and notify the patient's physician
- Nerve injuries can result in long-term pain, numbness, or loss of function
4. Hemolysis (Red Blood Cell Destruction)
Hemolysis is the #1 cause of specimen rejection in laboratories.
Causes of hemolysis:
| Cause | Explanation |
|---|---|
| Needle too small | Forces blood through a small opening, damaging cells |
| Pulling syringe plunger too fast | Creates excessive shear force on cells |
| Shaking tubes | Mechanical destruction of cells (always INVERT, never shake) |
| Forcing blood through a small needle | Syringe transfer through narrow gauge damages cells |
| Drawing from a hematoma | Damaged cells from the hematoma contaminate the specimen |
| Using alcohol that hasn't dried | Residual alcohol hemolysis cells on contact |
| Prolonged tourniquet application | Causes stasis and cell damage |
| Vigorous mixing of tubes | Rough handling destroys fragile cells |
Visual Sign: Hemolyzed serum/plasma appears pink to red (cherry-colored) instead of the normal straw-yellow color.
5. Accidental Arterial Puncture
Signs:
- Bright red blood (vs. dark red/maroon for venous blood)
- Pulsating blood flow — tube fills rapidly
- Patient may report unusual pain
Management:
- Remove the needle immediately
- Apply firm, direct pressure for at least 5 minutes (longer than venous bleeding)
- Do NOT apply a tourniquet — this can worsen bleeding
- Monitor the site after pressure release
- Document and report the incident
6. Petechiae
Petechiae are tiny, pinpoint red spots that appear on the skin (often above the tourniquet site).
Causes:
- Tourniquet too tight or applied too long
- Platelet disorders (thrombocytopenia)
- Fragile capillaries (common in elderly patients or those on anticoagulants)
The Two-Attempt Rule
Most facilities follow a two-attempt rule:
- If a phlebotomist is unsuccessful after two venipuncture attempts, they must stop and ask another qualified phlebotomist to try
- Making more than two attempts increases patient discomfort, anxiety, and risk of complications
- Document all failed attempts
- Never attempt a draw more than twice on the same patient
Failed Venipuncture: Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| No blood flow | Needle not in vein | Reposition slightly; do NOT probe excessively |
| Blood stops flowing | Vein collapsed, needle moved | Release tourniquet briefly, use smaller tube, reposition |
| Slow blood flow | Small vein, needle partially in vein | Ensure bevel is fully in the vein; try a smaller tube |
| Blood leaking around needle | Needle through both walls | Remove needle; apply pressure; try another site |
| Tube not filling | Loss of vacuum; tube expired | Try a new tube; check expiration date |
Vein Assessment Techniques
Proper vein assessment is a critical skill that improves first-stick success rates:
Palpation Technique
- Always use your index finger for palpation — NEVER your thumb (your thumb has its own pulse and you may confuse it with an artery)
- A healthy vein feels bouncy, resilient, and spongy — it compresses when pressed and rebounds when released
- An artery pulsates rhythmically — never puncture a pulsating vessel
- A tendon feels hard, cord-like, and does NOT rebound — tendons do not contain blood
Difficult-to-Find Veins
For patients with difficult-to-find veins, try these techniques:
| Technique | How It Helps |
|---|---|
| Warm the area | Apply a warm towel for 3-5 minutes to dilate veins |
| Lower the arm | Hang the arm below heart level to increase venous filling |
| Gently tap the vein | Light tapping stimulates vein dilation |
| Apply tourniquet | Restricts venous flow, causing veins to engorge |
| Ask patient to make a fist | Squeezing (NOT pumping) increases vein prominence |
| Use a vein finder | Infrared or near-infrared device to visualize veins through the skin |
| Try a blood pressure cuff | Inflate to diastolic pressure for more controlled venous engorgement |
Veins vs. Arteries vs. Tendons
| Characteristic | Vein | Artery | Tendon |
|---|---|---|---|
| Feel | Bouncy, resilient, spongy | Pulsating, firm | Hard, cord-like |
| Color | Bluish (visible through skin) | Not usually visible | Not colored |
| Compression | Compresses easily; rebounds | Rebounds forcefully | Does not compress |
| Location | Superficial to deep | Usually deeper than veins | Along bones and joints |
| Blood flow | Toward heart | Away from heart | N/A |
Documentation
Proper documentation is essential for every blood collection. The phlebotomist should document:
- Patient identification verification
- Date and time of collection
- Tests ordered and tubes collected
- Site used (e.g., right median cubital, left hand dorsal)
- Number of attempts (including failed attempts)
- Complications (hematoma, syncope, nerve pain, refusal)
- Disposition — specimen sent to lab, transported by (method)
- Phlebotomist signature/ID
Incident Reporting
- Any adverse event (needlestick, patient injury, syncope, nerve pain) must be documented in an incident report
- Include: date, time, description of event, patient and staff involved, actions taken, and follow-up plan
- Incident reports are used for quality improvement and are NOT punitive documents
Anticoagulant Therapy and Phlebotomy Considerations
Many patients seen by phlebotomists are on anticoagulant therapy. Understanding these medications helps ensure safe blood collection:
| Medication | Brand Name(s) | Mechanism | Lab Monitoring | Phlebotomy Impact |
|---|---|---|---|---|
| Warfarin | Coumadin | Inhibits vitamin K-dependent clotting factors | PT/INR | Apply extended pressure (5+ min); monitor for hematoma |
| Heparin (unfractionated) | Heparin IV | Inhibits thrombin and Factor Xa | aPTT | Apply extended pressure; draw from opposite arm of heparin drip |
| Enoxaparin | Lovenox | Inhibits Factor Xa | Anti-Xa levels (special test) | Apply extended pressure; subcutaneous injection sites may bruise easily |
| Rivaroxaban | Xarelto | Direct Factor Xa inhibitor | Usually not monitored | Apply extended pressure |
| Apixaban | Eliquis | Direct Factor Xa inhibitor | Usually not monitored | Apply extended pressure |
| Dabigatran | Pradaxa | Direct thrombin inhibitor | Usually not monitored | Apply extended pressure |
| Aspirin | Various | Inhibits platelet aggregation | Bleeding time (rarely) | Apply extended pressure; may bruise easily |
| Clopidogrel | Plavix | Inhibits platelet aggregation | Platelet function tests | Apply extended pressure |
Critical Clinical Tip: Always ask patients if they are taking any blood thinners or anticoagulants before the draw. Apply firm pressure for at least 5 minutes (or longer) after the venipuncture to prevent hematoma formation. DO NOT apply a bandage until bleeding has completely stopped.
What is the MOST COMMON complication of venipuncture?
During a venipuncture, the patient reports a sharp, shooting, electric-like pain radiating down their arm. The phlebotomist should:
Which of the following is the #1 cause of specimen rejection in laboratories?
Which of the following can cause hemolysis of a blood specimen? (Select all that apply)
Select all that apply
A phlebotomist has made two unsuccessful venipuncture attempts on a patient. What should they do next?
Bright red, pulsating blood during a venipuncture most likely indicates:
After venipuncture, firm pressure should be applied to the puncture site for at least ___ minutes to prevent hematoma formation.
Type your answer below
A patient begins to feel lightheaded and nauseated during a blood draw. The phlebotomist's FIRST action should be:
Match each venipuncture complication to its primary identifying sign:
Match each item on the left with the correct item on the right
A patient on warfarin (Coumadin) has blood drawn. How long should the phlebotomist hold pressure after removing the needle?
While palpating for a vein, the phlebotomist feels a structure that pulsates rhythmically. This structure is most likely:
The phlebotomist should always use their _____ finger (not their thumb) to palpate veins.
Type your answer below
During a venipuncture, the blood suddenly stops flowing into the tube. Which of the following is the MOST appropriate first action?
Which of the following patients requires PHYSICIAN AUTHORIZATION before drawing blood from a foot vein?
Which of the following techniques can help locate veins in a patient with difficult-to-find veins? (Select all that apply)
Select all that apply