Key Takeaways

  • Cleaning removes dirt; disinfection kills germs; sterilization kills ALL microorganisms
  • Always clean before disinfecting - dirt blocks disinfectants from working
  • Use bleach-based products for C. diff rooms - other disinfectants don't kill the spores
  • High-touch surfaces (bed rails, call lights, doorknobs) need frequent disinfection
  • Contact time matters - disinfectant must remain wet for the required time to work
Last updated: January 2026

Cleaning, Disinfection, and Sterilization

Proper cleaning and disinfection of patient care equipment and the environment is essential for preventing the spread of infection. CNAs play a key role in maintaining a clean and safe healthcare environment.

Definitions: Know the Difference

TermDefinitionLevel of Killing
CleaningRemoving visible dirt and debrisReduces germs, doesn't kill all
DisinfectionKilling most germs on surfacesKills most pathogens
SterilizationKilling ALL microorganismsEliminates 100% of germs

When Each Level is Required

LevelUsed ForExamples
CleaningNon-critical items, general environmentBed frames, floors, furniture
Low-level disinfectionSurfaces that touch intact skinStethoscopes, BP cuffs
High-level disinfectionSemi-critical itemsRespiratory equipment
SterilizationCritical items entering sterile body areasSurgical instruments (not CNA scope)

Classification of Patient Care Items

CategoryContact TypeProcess RequiredExamples
CriticalEnters sterile tissue or bloodSterilizationSurgical instruments, needles
Semi-criticalContacts mucous membranesHigh-level disinfectionRespiratory equipment, scopes
Non-criticalContacts intact skin onlyLow-level disinfectionBP cuffs, stethoscopes, bed rails

Cleaning Principles

Basic cleaning rules:

  • Clean before disinfecting (dirt blocks disinfectant)
  • Clean from cleanest to dirtiest areas
  • Clean from top to bottom
  • Clean from back to front
  • Use appropriate cleaning solutions
  • Follow manufacturer's directions

Common Disinfectants in Healthcare

DisinfectantEffective AgainstCommon Uses
Quaternary ammoniumMany bacteriaGeneral surface disinfection
Chlorine (bleach)Most pathogens including C. diffC. diff rooms, blood spills
Hydrogen peroxideBroad spectrumEnvironmental surfaces
Alcohol (70%)Many bacteria/virusesEquipment, small surfaces
PhenolicsBroad spectrumFloors, surfaces

C. diff reminder: Use bleach-based products - many disinfectants don't kill C. diff spores!

Environmental Cleaning

Daily cleaning responsibilities may include:

AreaCleaning Focus
Bed railsHigh-touch surface - disinfect frequently
Overbed tablesWipe before and after meals
Call lightsHigh-touch - clean multiple times daily
DoorknobsHigh-touch surfaces
Light switchesOften overlooked high-touch area
Bathroom surfacesClean after each use if possible
Patient equipmentClean between patients

High-Touch Surfaces

Surfaces touched frequently need more attention:

  • Bed rails
  • Call lights
  • Telephone
  • Remote controls
  • Light switches
  • Door handles
  • Chair arms
  • IV poles (handles)
  • Toilet flush handles
  • Faucet handles

Terminal Cleaning

Terminal cleaning occurs when a patient is discharged or transferred:

StepAction
1Remove all trash and linens
2Clean all surfaces (top to bottom)
3Disinfect all surfaces
4Clean and disinfect bathroom
5Make bed with clean linens
6Replace supplies
7Allow room to air out if needed

Equipment Cleaning

Between patients:

  • Clean and disinfect all reusable equipment
  • Check manufacturer's instructions
  • Allow proper contact time for disinfectant
  • Never skip this step

CNA-cleaned equipment often includes:

  • Blood pressure cuffs
  • Stethoscopes
  • Thermometers
  • Wheelchairs
  • Shower chairs
  • Bedpans and urinals

Safe Handling of Cleaning Products

Safety MeasureReason
Read labelsKnow proper use and hazards
Wear glovesProtect skin
Don't mix productsChemical reactions can be dangerous
Adequate ventilationAvoid fume inhalation
Store properlyAway from patient care areas
Report skin irritationMay need different product

Contact Time

Contact time (dwell time) is how long a disinfectant must remain wet on a surface to be effective:

  • Read product label for required time
  • Surface must stay wet for full contact time
  • May need to reapply if dries too quickly
  • Wiping too soon reduces effectiveness
Test Your Knowledge

What is the difference between cleaning and disinfection?

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Test Your Knowledge

Which disinfectant should be used for a room where a patient with C. diff was staying?

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Test Your Knowledge

What is "contact time" in disinfection?

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